Tissue culture studies in bovine leukosis

• Published in: Acta veterinaria scandinavica Vol. 11; no. 1; pp. 103 - 113
• Main Author: Hugoson, G
• Format: Journal Article
• Language: English
• Published: England BioMed Central 01.01.1970
• Subjects: animal science; Animals; Animals, Newborn; Cattle; Cattle Diseases - pathology; Culture Techniques; Lymph Nodes - pathology; Lymphatic Diseases - immunology; Lymphatic Diseases - pathology; Lymphatic Diseases - veterinary; zoology
• ISSN: 0044-605X; 1751-0147; 1751-0147
• DOI: 10.1186/BF03548007

By examining cover slips stained at regular intervals the development of primary lymph node tissue cultures from 2 cases of juvenile bovine leukosis and 2 “normal” foetuses was studied. Secondly, comparisons were made between cell lines prepared from 23 bovines — “normal” animals and foetuses, cases of adult leukosis, juvenile leukosis and skin leukosis — with respect to susceptibility to infection with interferon sensitive viruses (VSV and PRV) and/or growth rate in the presence of sera from “normal” and leukotic animals. Nuclear budding, nuclear fragmentation, lymphocyte adsorption and giant cell formation were observed — though to a much greater extent in cultures prepared from the leukosis animals — in all 4 cases studied. No indications of different susceptibility for the test virus infections appeared between cell lines prepared from “normal” and leukotic animals. The growth rate of the cell lines seemed similar irrespective of the kind of serum used.