Trypan Blue Exclusion Test of Cell Viability

The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cell...

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Bibliographic Details
Published inCurrent protocols in immunology Vol. 111; p. A3.B.1
Main Author Strober, Warren
Format Journal Article
LanguageEnglish
Published United States 01.11.2015
Subjects
Animals
Cell Survival
Cells, Cultured
Cytoplasm - metabolism
Humans
Microscopy
Staining and Labeling - methods
Trypan Blue - chemistry
dye exclusion
trypan blue
cell viability
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Summary:The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).
ISSN:1934-368X
DOI:10.1002/0471142735.ima03bs111