Enhanced peripheral nerve regeneration through asymmetrically porous nerve guide conduit with nerve growth factor gradient

In this study, we fabricated a nerve guide conduit (NGC) with nerve growth factor (NGF) gradient along the longitudinal direction by rolling a porous polycaprolactone membrane with NGF concentration gradient. The NGF immobilized on the membrane was continuously released for up to 35 days, and the re...

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Published inJournal of biomedical materials research. Part A Vol. 106; no. 1; pp. 52 - 64
Main Authors Oh, Se Heang, Kang, Jun Goo, Kim, Tae Ho, Namgung, Uk, Song, Kyu Sang, Jeon, Byeong Hwa, Lee, Jin Ho
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.01.2018
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Summary:In this study, we fabricated a nerve guide conduit (NGC) with nerve growth factor (NGF) gradient along the longitudinal direction by rolling a porous polycaprolactone membrane with NGF concentration gradient. The NGF immobilized on the membrane was continuously released for up to 35 days, and the released amount of the NGF from the membrane gradually increased from the proximal to distal NGF ends, which may allow a neurotrophic factor gradient in the tubular NGC for a sufficient period. From the in vitro cell culture experiment, it was observed that the PC12 cells sense the NGF concentration gradient on the membrane for the cell proliferation and differentiation. From the in vivo animal experiment using a long gap (20 mm) sciatic nerve defect model of rats, the NGC with NGF concentration gradient allowed more rapid nerve regeneration through the NGC than the NGC itself and NGC immobilized with uniformly distributed NGF. The NGC with NGF concentration gradient seems to be a promising strategy for the peripheral nerve regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 52–64, 2018.
Bibliography:These authors contributed equally to this work.
ObjectType-Article-1
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ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.36216