Raman and surface-enhanced Raman spectroscopy on horns and their correlation with biomechanical properties
Recently horns are considered as potential candidates for various biomedical applications because of their distinct biomechanical properties. We therefore performed Raman spectral analysis and mechanical test on the horns of buffalo, cattle and sheep to obtain information about the molecular composi...
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Published in | Journal of Raman spectroscopy Vol. 47; no. 8; pp. 926 - 932 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Bognor Regis
Blackwell Publishing Ltd
01.08.2016
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Recently horns are considered as potential candidates for various biomedical applications because of their distinct biomechanical properties. We therefore performed Raman spectral analysis and mechanical test on the horns of buffalo, cattle and sheep to obtain information about the molecular compositions in various parts of the horns and their correlation with biomechanical properties. We also developed a surface enhanced Raman spectroscopy (SERS) to trace their degradation products. We found that various parts of the horns have similar overall molecular compositions, but have differences in the bands of C–C–S–S–C–C conformation, α‐helix and β‐sheet conformation, especially in the bands of 507–515 cm−1 which reflect the relative sulfur content and the structural conformation of the disulfide linkages. The Raman intensities at the region are highly correlated with the Young's modulus and tensile strength of the horns, indicating that the biomechanical properties of the horns are mainly determined by their disulfide bonds. It also suggests that Raman spectroscopy has the potential to perform non‐destructive detection on the mechanical strength of horns. The SERS measurements verified that the main constituents of the degradation products from both the buffalo and sheep horns were amino acids and polypeptides, suggesting that the horns are suitable candidates for biomaterial scaffolds. Copyright © 2016 John Wiley & Sons, Ltd.
We performed Raman spectral analysis and mechanical test on the horns of buffalo, cattle and sheep, and developed a surface enhanced Raman spectroscopy to trace their degradation products. We found that the three horns have similar overall molecular compositions, but have differences in the bands of C–C–S–S–C–C conformation, α‐helix and β‐sheet conformation. The Raman intensities are highly correlated with the Young's modulus and tensile strength of the horns. The main constituents of their degradation products were amino acids and polypeptides. |
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Bibliography: | Supporting info item Guang Dong Provincial Science and Technology Foundation ark:/67375/WNG-MK0Q4P0F-5 ArticleID:JRS4926 istex:17CA2C1EE2D422D9EC6FBF1FD81C2F501B4388BB Chinese National Natural Science Foundation - No. 30940019; No. 60377043 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0377-0486 1097-4555 |
DOI: | 10.1002/jrs.4926 |