p35 interacts with α-tubulin and organelle proteins: Nuclear translocation of p35 in dying cells

• Published in: Proteomics (Weinheim) Vol. 9; no. 16; pp. 4036 - 4047
• Main Authors: Son, Yonghae, Kim, Sunmi, Choi, Kyungha, Park, Youngchul, Eo, Seongkug, Kim, Youngkook, Rhim, Byungyong, Kim, Koanhoi
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01.08.2009; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Amino Acid Motifs; Analytical, structural and metabolic biochemistry; Animals; Apoptosis; Biological and medical sciences; Blotting, Western; Cell Line; Cell Nucleus - metabolism; CHO Cells; Chromatography, Affinity; Cricetinae; Cricetulus; Dactinomycin - pharmacology; Fundamental and applied biological sciences. Psychology; Heterogeneous Nuclear Ribonucleoprotein A1; Heterogeneous-Nuclear Ribonucleoprotein Group A-B - metabolism; Heterogeneous-Nuclear Ribonucleoprotein Group C - metabolism; hnRNPs; Humans; Microscopy, Confocal; Miscellaneous; p35; Protein arrays; Protein Binding; Protein Transport - drug effects; Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; TOM40; Tubulin - metabolism; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; α‐Tubulin; α-Tubulin; Ribonucleoprotein; Protein arrays; Tubulin; p35; Cell death; Proteomics; hnRNPs; TOM40; Nuclear protein; Apoptosis
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200900122

We identified heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2, hnRNP A1, the translocase of the transporter outer membrane 40 (TOM40), and α-tubulin as new interaction partners of anti-apoptotic protein p35 using MS-based functional proteomics with GST-p35 fusion protein as a bait, and using a pull-down assay with p35-6His followed by Western blot analysis. p35 was localized in the cytoplasm and in distinct organelles such as the nucleus and mitochondria. p35 was more abundant in the cytoplasm than it was in the nucleus. It co-localized with α-tubulin in the cytoplasm in the absence of a death stimulus. However, while cells were undergoing death induced by actinomycin D, cytoplasmic p35 was translocated into the nucleus; this process was inhibited by deletions of the N- and C-terminal domains containing leucine-rich motifs. Gene delivery of p35 using recombinant adenoviruses inhibited cytoplasmic compartmentalization of hnRNP C1/C2 and hnRNP A1 in dying cells. This study demonstrated translocation of p35 into the nuclei, as well as protection of the hnRNPs from redistribution in cells undergoing death. We propose an active role for p35 in maintaining the integrity of nuclear proteins during cell death.