Evaluation of on-line solid phase extraction-capillary electrophoresis-electrospray-mass spectrometry for the analysis of neuropeptides in human plasma
In this study, the suitability of SPE coupled on-line to CE-electrospray-MS (SPE-CE-ESI-MS) was evaluated for the analysis of neuropeptides in human plasma. First, CE-ESI-MS was investigated and a sample pretreatment based on precipitation with ACN was used for cleanup of plasma samples. The main qu...
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Published in | Electrophoresis Vol. 29; no. 16; pp. 3366 - 3376 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
Wiley-VCH Verlag
01.08.2008
WILEY-VCH Verlag WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | In this study, the suitability of SPE coupled on-line to CE-electrospray-MS (SPE-CE-ESI-MS) was evaluated for the analysis of neuropeptides in human plasma. First, CE-ESI-MS was investigated and a sample pretreatment based on precipitation with ACN was used for cleanup of plasma samples. The main quality parameters were determined and were consistent with those previously obtained for the analysis of standard mixtures, e.g., the LODs were of around 1 μg/mL. SPE-CE-ESI-MS employing microcartridges containing a C18 stationary phase was explored in order to decrease the LODs. A double-step sample cleanup pretreatment consisting of precipitation with ACN and ultrafiltration through 3000 Da MW cut-off membranes was needed to prevent the microcartridge saturation. Repeatability, reproducibility, linearity, and LODs of the SPE-CE-ESI-MS method and the durability of the microcartridges were studied. The LODs were markedly improved, ranging between 10 and 0.1 ng/mL depending on the peptide. |
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Bibliography: | http://dx.doi.org/10.1002/elps.200700872 Spanish Ministry of Education and Science for a FPU (Formación del Profesorado Universitario) fellowship Spanish government - No. CTQ2005-04357/BQU istex:3CA0361CB15C72143ACFD06CA5E79A00EAF52E22 ark:/67375/WNG-FSBHMTML-3 ArticleID:ELPS200700872 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200700872 |