Tandem MS Characterization of Endosperm Lipid Profile in Isogenic Waxy Wheat Versus Wildtype Parent Cultivars

• Published in: Journal of the American Oil Chemists' Society Vol. 93; no. 6; pp. 751 - 756
• Main Authors: Brewer, Lauren R., Wetzel, David L.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2016; Springer Nature B.V
• Subjects: Agriculture; Analysis; Biomaterials; Biotechnology; breadmaking quality; Chemistry; Chemistry and Materials Science; Cultivars; endosperm; Food Science; Genetic lipid alteration; hexaploidy; Industrial Chemistry/Chemical Engineering; Lipid analysis; Lipids; Mass spectrometry; Original Paper; Tandem mass spectrometry; triacylglycerols; Unfractionated extract; Waxy wheat; Wheat; Tandem mass spectrometry; Unfractionated extract; Waxy wheat; Lipids; Genetic lipid alteration; Lipid analysis
• ISSN: 0003-021X; 1558-9331
• DOI: 10.1007/s11746-016-2823-8

Among bread quality wheat cultivars, the percentage of lipids is minor, but its potential contribution to desirable traits is significant; particularly the glycolipid fraction. The lipid profile of total lipid extracts from isogenic waxy wheat kernels reveals the genetic expression compared with extracts from the corresponding wildtype wheat kernels. The collateral enhancement of desirable lipid enhancement of waxy wheats that accompanies the genetically defined carbohydrate difference is the subject of the analytical efforts described in this paper. Tandem mass spectrometry directly applied to total extract enables identification and measurement of acyl group populations within each lipid class. Significant increases were observed in glycolipid content for waxy wheats and the N11 waxy (hexaploid) triglycerides were significantly increased vs the wildtype parent.