Manganese peroxidase production from cassava residue by Phanerochaete chrysosporium in solid state fermentation and its decolorization of indigo carmine

• Published in: Chinese journal of chemical engineering Vol. 23; no. 1; pp. 227 - 233
• Main Authors: Li, Huixing, Zhang, Ruijing, Tang, Lei, Zhang, Jianhua, Mao, Zhonggui
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2015
• Subjects: Cassava; Cassava residue; Decoloring; Fermentation; Indigo; Indigo carmine; Manganese; Manganese peroxidase; Manihot esculenta; Phanerochaete chrysosporium; Residues; Solid state; Solid state fermentation; 固态发酵生产; 木薯渣; 脱色处理; 超高效液相色谱; 锰过氧化物酶; 靛蓝; 高附加值产品; 黄孢原毛平革菌; Cassava residue; Manganese peroxidase; Indigo carmine; Phanerochaete chrysosporium; Solid state fermentation
• ISSN: 1004-9541; 2210-321X
• DOI: 10.1016/j.cjche.2014.11.001

Bioconversion of lignocellulosic wastes to higher value products through fungal fermentation has economic and ecological benefits. In this study, to develop an effective strategy for production of manganese peroxidase（Mn P）from cassava residue by Phanerochaete chrysosporium in solid state fermentation, the stimulators of Mn P production were screened and their concentrations were optimized by one-at-a-time experiment and Box–Behnken design. The maximum Mn P activity of 186.38 nkat·g-1dry mass of the sample was achieved after 6 days of fermentation with the supplement of 79.5 mmol·L-1·kg-1acetic acid, 3.21 ml·kg-1soybean oil, and 28.5 g·kg-1alkaline lignin, indicating that cassava residue is a promising substrate for Mn P production in solid state fermentation. Meanwhile, in vitro decolorization of indigo carmine by the crude Mn P was also carried out, attaining the ratio of 90.18% after 6 h of incubation. An oxidative mechanism of indigo carmine decolorization by Mn P was proposed based on the analysis of intermediate metabolites with ultra-high performance liquid chromatography and gas chromatography tandem mass spectrometry. Using the crude Mn P produced from cassava residue for indigo carmine decolorization gives an effective approach to treat dyeing effluents.