Development of Cre–loxP technology in zebrafish to study the regulation of fish reproduction

• Published in: Fish physiology and biochemistry Vol. 39; no. 6; pp. 1525 - 1539
• Main Authors: Lin, Heng-Ju, Lee, Shu-Hua, Wu, Jen-Leih, Duann, Yeh-Fang, Chen, Jyh-Yih
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.12.2013; Springer Netherlands; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; animal ovaries; Animal Physiology; Animals; Animals, Genetically Modified - physiology; anti-Mullerian hormone; Apoptosis; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Biomedical and Life Sciences; Calibration; Cytomegalovirus; Danio rerio; Female; Fish; Freshwater & Marine Ecology; Gene Expression Regulation; gene overexpression; Gene Transfer Techniques; Genes; Genomes; Histology; Infertility; Integrases - genetics; Life Sciences; Male; marketing; Morphology; plasmids; Reproduction; Salmon; Stem cells; Zebrafish - physiology; zona pellucida; Zoology; Taiwan; Transgene fish; Zebrafish; Infertility; Cre–loxP technology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-013-9806-6

One cannot seek permission to market transgenic fish mainly because there is no field test or any basic research on technological developments for evaluating their biosafety. Infertility is a necessary adjunct to exploiting transgenic fish unless completely secure land-locked facilities are available. In this study, we report the generation of a Cre transgenic zebrafish line using a cytomegalovirus promoter. We also produced fish carrying the Bax1 and Bax2 plasmids; these genes were separated by two loxP sites under a zona pellucida C promoter or were driven by an anti-Müllerian hormone promoter. We inserted a red fluorescent protein gene between the two loxP sites. After obtaining transgenic lines with the two transgenic fish crossed with each other (Cre transgenic zebrafish x loxP transgenic zebrafish), the floxed DNA was found to be specifically eliminated from the female or male zebrafish, and apoptosis gene expressions caused ovarian and testicular growth cessation and degeneration. Overexpression of the Bax1 and Bax2 genes caused various expression levels of apoptosis-related genes. Accordingly, this transgenic zebrafish model system provides a method to produce infertile fish and may be useful for application to genetically modified fish.