Reduction of Salmonella Typhimurium by Fermentation Metabolites of Diamond V Original XPC in an In Vitro Anaerobic Mixed Chicken Cecal Culture
Fermentation metabolites of Diamond V Original XPC™ (XPC), a biological product derived from yeast fermentation, were evaluated for their ability to reduce the Typhimurium population using an mixed anaerobic culture system containing cecal microbiota to simulate chicken hindgut conditions. Four diff...
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Published in | Frontiers in veterinary science Vol. 3; p. 83 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
16.09.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Fermentation metabolites of Diamond V Original XPC™ (XPC), a biological product derived from yeast fermentation, were evaluated for their ability to reduce the
Typhimurium population using an
mixed anaerobic culture system containing cecal microbiota to simulate chicken hindgut conditions. Four different samples were prepared: anaerobic mixed culture containing (1) feed only, (2) cecal only (ceca were harvested from 42 days old broiler chickens), (3) feed and cecal contents, and (4) feed, cecal contents, and 1% XPC. Two experimental conditions were investigated: Group 1, in which the cecal content was added at the same time as a
. Typhimurium marker strain and Group 2, in which the cecal content was preincubated for 24 h prior to the inoculation with the
. Typhimurium marker strain. The mixed cultures were incubated anaerobically at 37°C, and the
. Typhimurium marker strain was enumerated at 0, 24, and 48 h. Analysis of short chain fatty acids was also conducted for 24 h. In the Group 1 experiment, adding XPC did not exhibit significant reduction of
. Typhimurium. However, the presence of XPC resulted in rapid reduction of
. Typhimurium in Group 2.
. Typhimurium was reduced from 6.81 log
CFU/ml (0 h) to 3.73 log
CFU/ml and 1.19 log
CFU/ml after 24 and 48 h, respectively. These levels were also 2.47 log
and 2.72 log
lower than the
. Typhimurium level recovered from the control culture with feed and cecal contents, but without XPC. Based on these results, it appears that the ability of XPC to reduce
. Typhimurium requires the presence of the cecal microbiota. Short chain fatty acid analysis indicated that acetate and butyrate concentrations of cultures containing XPC were twofold greater than the control cultures by 24 h of anaerobic growth. Results from the present study suggest that dietary inclusion of XPC may influence cecal microbiota fermentation and has the potential to reduce
in the cecum. Implications of these findings suggest that XPC may decrease preharvest levels of
in broilers and layers. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Specialty section: This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science Reviewed by: Lisa Bielke, Ohio State University, USA; Michael J. Rothrock, United States Department of Agriculture, USA Edited by: Ryan Arsenault, University of Delaware, USA |
ISSN: | 2297-1769 2297-1769 |
DOI: | 10.3389/fvets.2016.00083 |