Resistance of paramyxoviridae to type I interferon-induced Bos taurus Mx1 dynamin

• Published in: Journal of interferon & cytokine research Vol. 25; no. 4; pp. 192 - 201
• Main Authors: Leroy, Michael, Baise, Etienne, Pire, Grégory, Gérardin, Joel, Desmecht, Daniel
• Format: Journal Article Web Resource
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.04.2005; Mary Ann Liebert Inc
• Subjects: Animals; Antiviral Agents - metabolism; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Bos taurus; Cattle - metabolism; Cattle - virology; Cercopithecus aethiops; Cytopathogenic Effect, Viral; Dynamins - metabolism; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; Human health sciences; Immunologie & maladie infectieuse; Immunology & infectious disease; interferon; Interferon Type I - physiology; Life sciences; Myxovirus Resistance Proteins; Paramyxoviridae; Paramyxoviridae - physiology; Paramyxovirus; Sciences de la santé humaine; Sciences du vivant; Vero Cells; Vesicular stomatitis virus; Viral Proteins - analysis; Viral Proteins - metabolism
• ISSN: 1079-9907; 1557-7465; 1557-7465
• DOI: 10.1089/jir.2005.25.192

Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae family. Recently, bovine Mx1 protein (BoMx1) was included in the group of Mx proteins with authenticated antiviral potential, as it dramatically represses the replication of vesicular stomatitis virus (VSV). As replication in bovine cells of Pi3, respiratory syncytial (RS), and Sendai (Se) viruses, all members of the same family, is known to be reduced on IFN-alpha incorporation into the culture medium, it was hypothesized that the BoMx1 pathway possibly was involved, its antiviral spectrum thus probably extending to Paramyxoviridae. In this study, probing of BoMx1-inhibiting effects was carried out by infecting a transgenic Vero cell line that allows tightly regulated conditional expression of BoMx1 after doxycycline treatment with a wide array of Paramyxoviridae. Expressing and nonexpressing cells displayed similar viability, cytopathic effects (CPEs), and amounts of infectious virus yields, whatever the infecting virus or the multiplicity of infection (moi) imposed. It is, therefore, concluded that BoMx1 does not interfere with Paramyxoviridae.