Determination of 17 β-Estradiol in Rabbit Plasma by Gas Chromatography with Flame Ionization Detection

• Published in: Indian journal of pharmaceutical sciences Vol. 74; no. 3; pp. 248 - 253
• Main Authors: Yilmaz, B, Kadioglu, Y
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt Ltd 01.05.2012
• Subjects: Research Paper; pharmacokinetics; Flame ionization detection; liquid–liquid extraction; gas chromatography; 17 β-estradiol; validation
• ISSN: 0250-474X; 1998-3743
• DOI: 10.4103/0250-474X.106067

This article describes gas chromatography-flame ionization detection method for determination of 17 β-estradiol in rabbit plasma. 17 β-estradiol and internal standard progesterone were extracted from plasma using liquid-liquid extraction method. Linearity was found between 0.25 and 20 μg/ml (r(2)=0.994) for plasma samples. Intra-day and inter-day precision, expressed as the relative standard deviation were less than 5.5%, and accuracy (relative error) was less than 3.5%. The mean recovery of 17 β-estradiol samples was 94.4%. The limits of detection and quantification of method for plasma samples were 0.10 μg/ml and 0.15 μg/ml, respectively. Also, clinically used other 10 drugs were investigated to check for potential interferences and the method was successfully applied to the determination of 17 β-estradiol in New Zealand white rabbits.