Dietary organic selenium improves growth, survival and resistance to Vibrio mimicus in cultured marron, Cherax cainii (Austin, 2002)

• Published in: Fish & shellfish immunology Vol. 35; no. 1; pp. 79 - 85
• Main Authors: Nugroho, Rudy Agung, Fotedar, Ravi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2013
• Subjects: Animal Feed - analysis; Animals; Aquaculture; Astacoidea - drug effects; Astacoidea - growth & development; Astacoidea - immunology; Astacoidea - physiology; Dietary Supplements - analysis; Growth and health; Immunity, Innate; Marron; Organic selenium; Organoselenium Compounds - administration & dosage; Vibrio mimicus; Vibrio mimicus - physiology; Marron; Vibrio mimicus; Growth and health; Organic selenium
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2013.04.011

To determine the effects of dietary organic selenium (OS) supplementation on the growth performance and immune competence of marron, Cherax cainii (Austin, 2002), a group of marron were fed 0.2 g kg−1 of Sel-Plex® supplemented basal diet and then compared with another group (control) of marron fed basal diet without any supplementation. After 90 days of feeding, final weight, average weekly gains (AWG), relative gain rate (RGR), specific growth rate (SGR), survival, total and differential haemocyte counts (THC and DHC), were compared between the two groups. Surviving marron from each group were then divided into three sub-groups (three tanks per sub-group with seven marron per tank); (1) first sub-group was injected with 20 μL of 3.24 × 106 cfu Vibrio mimicus; (2) the second sub-group was injected with 20 μL normal saline and (3) the third sub-group was not subjected to injection and became the control group. THC, DHC, neutral red retention time (NRRT) and Vibrio ranks of post-injected marron were evaluated for 96 h, at every 24-h interval. The results showed that after 90 days of feeding, final weight, AWG, RGR, SGR, survival, THC, proportion of hyaline cells of OS-fed marron were significantly higher (P < 0.05) than the control group, whereas proportion of granular and semigranular cells were not affected by dietary OS. After challenging with V. mimicus, survival rate of marron without dietary OS significantly decreased (P < 0.05) as compared to the control group of marron. THC of marron in all sub-groups were significantly reduced (P < 0.05) after the challenge. However, THC and granular cells of sub-groups fed OS were higher than other sub-groups. Vibrio ranks and NRRT of marron fed OS were significantly lower and slower, respectively, than marron fed without OS. These findings demonstrated the benefits of OS inclusion in the marron diet in terms of growth, health and disease resistance. •Organic selenium supplementation improved growth and immune performance of marron.•Proportion of hyaline cells affected by organic selenium supplementation.•Organic selenium in the diet improved total haemocyte count and resistance to pathogen.•Organic selenium supplementation reduced Vibrio rank of marron after infection.•Lysosomal membrane more stable on marron fed organic selenium than control.