Transcriptome profiling of human papillary and reticular fibroblasts from adult interfollicular dermis pinpoints the ‘tissue skeleton’ gene network as a component of skin chrono-ageing

• Published in: Mechanisms of ageing and development Vol. 179; pp. 60 - 77
• Main Authors: Haydont, Valérie, Neiveyans, Véronique, Fortunel, Nicolas O., Asselineau, Daniel
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.04.2019
• ISSN: 0047-6374; 1872-6216; 1872-6216
• DOI: 10.1016/j.mad.2019.01.003

•This work is about dermal Fp and Fr fibroblasts from young and old human skin.•Age-related biomarkers were identified by genome-wide transcriptome profiling.•Ageing affects ‘tissue skeleton’ transcripts levels, even more in Fr than in Fp.•Nuclear circularity decreases with ageing in both Fp and Fr dermal fibroblasts. Interactions between extracellular matrix (ECM) and fibroblasts are essential for maintaining dermis integrity, and are subject to ageing. The ötissue skeleton’ network connects ECM to the nucleus and DNA, impacting nuclear shape and gene expression. In a previous Mech Ageing Dev publication, we have presented a transcriptomic study of papillary (Fp) and reticular (Fr) fibroblasts, with a main focus on Fp ageing. As shown here, ageing affects ötissue skeleton’ transcripts, even more clearly in Fr than in Fp. Accordingly, using circular index measurement, we show that nuclear shape is affected by ageing in both cell fractions.