Metabolic and cellular stress responses of catfish, Horabagrus brachysoma (Günther) acclimated to increasing temperatures

• Published in: Journal of thermal biology Vol. 65; pp. 32 - 40
• Main Authors: Dalvi, Rishikesh S., Das, Tilak, Debnath, Dipesh, Yengkokpam, Sona, Baruah, Kartik, Tiwari, Lalchand R., Pal, Asim K.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2017; Elsevier BV
• Subjects: Acclimation; Acclimatization; Adenosine triphosphatase; Animals; Ascorbic acid; Blood Glucose - analysis; Blood Glucose - metabolism; Carbohydrate Metabolism; Catfish; Catfishes - blood; Catfishes - physiology; Cellular stress response; Energy Metabolism; Enzymes; Fish Proteins - analysis; Fish Proteins - metabolism; Gluconeogenesis; Glycolysis; High temperature; Homeostasis; Horabagrus brachysoma; Hot Temperature; HSP70; HSP70 Heat-Shock Proteins - analysis; HSP70 Heat-Shock Proteins - metabolism; Hsp70 protein; Lipid Metabolism; Metabolic enzymes; Metabolic Networks and Pathways; Metabolism; Pentose phosphate pathway; Protein turnover; Secondary stress responses; Stress response; Stress, Physiological; Thermal acclimation; Tricarboxylic acid cycle; NADPH; AST; RT; HSP70; FBPase; ALP; ATPase; G6PDH; Horabagrus brachysoma; ALT; Thermal acclimation; ACP; MDH; Secondary stress responses; LDH; SDS-PAGE; G6Pase; ROS; Metabolic enzymes
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/j.jtherbio.2017.02.003

We investigated the metabolic and cellular stress responses in an endemic catfish Horabagrus brachysoma acclimated to ambient (26°C), 31, 33 and 36°C for 30 days. After acclimation, fish were sampled to investigate changes in the levels of blood glucose, tissue glycogen and ascorbic acid, activities of enzymes involved in glycolysis (LDH), citric acid cycle (MDH), gluconeogenesis (FBPase and G6Pase), pentose phosphate pathway (G6PDH), protein metabolism (AST and ALT), phosphate metabolism (ACP and ALP) and energy metabolism (ATPase), and HSP70 levels in various tissues. Acclimation to higher temperatures (33 and 36°C) significantly increased activities of LDH, MDH, ALP, ACP, AST, ALT and ATPase and blood glucose levels, whereas decreased the G6PDH enzyme activity and, tissue glycogen and ascorbic acid. Results indicated an overall increase in the carbohydrate, protein and lipid metabolism implying increased metabolic demands for maintaining homeostasis in fish acclimated to higher temperatures (33 and 36°C). We observed tissue specific response of HSP70 in H. brachysoma, with significant increase in gill and liver at 33 and 36°C, and in brain and muscle at 36°C, enabling cellular protection at higher acclimation temperatures. In conclusion, H. brachysoma adjusted metabolic and cellular responses to withstand increased temperatures, however, these responses suggest that the fish was under stress at 33°C or higher temperature. •We investigated metabolic and cellular stress responses in Horabagrus brachysoma acclimated at 26, 31, 33 and 36°C for 30 days.•Acclimation to 33 and 36°C enhanced carbohydrate, protein and lipid metabolism.•Elevated levels and tissue specific variations of HSP70 enable cellular protection at 33 and 36°C.