Gordonibacter pamelaeae gen. nov., sp. nov., a new member of the Coriobacteriaceae isolated from a patient with Crohn's disease, and reclassification of Eggerthella hongkongensis Lau et al. 2006 as Paraeggerthella hongkongensis gen. nov., comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 6; pp. 1405 - 1415
• Main Authors: Wurdemann, Dieco, Tindall, Brian J, Pukall, Rudiger, Lunsdorf, Heinrich, Strompl, Carsten, Namuth, Theresa, Nahrstedt, Hannes, Wos-Oxley, Melissa, Ott, Stephan, Schreiber, Stephan, Timmis, Kenneth N, Oxley, Andrew P. A
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.06.2009; Society for General Microbiology
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Colon - microbiology; Crohn Disease - microbiology; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Genotype; Gram-Positive Bacterial Infections - microbiology; Humans; Microbiology; Molecular Sequence Data; Phenotype; Phylogeny; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Systematics; Taxonomic review; Crohn disease; New genus; Taxonomy; Bacteria; New species; Clinical isolate
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.005900-0

1 Environmental Microbiology Laboratory, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany 2 DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, D-38124 Braunschweig, Germany 3 Department of Vaccinology, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany 4 Biodegradation Group, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany 5 Institute for Clinical Molecular Biology, Christian-Albrechts-University Kiel, Schittenhelmstr. 12, D-24105 Kiel, Germany Correspondence Dieco Würdemann dieco.wuerdemann{at}helmholtz-hzi.de A strictly anaerobic, Gram-positive, short-rod/coccobacillus-shaped bacterial strain, designated 7-10-1-b T , was isolated from the colon of a patient suffering from acute Crohn's disease. The isolate formed small, pale-white, semi-translucent colonies on solid cultivation media. The strain was catalase-positive and metabolized only a small number of carbon sources. Whole-cell fatty acids consisted predominantly of saturated fatty acids (89 %), of which 15 : 0 anteiso was the major component. The polar lipids phosphatidylglycerol and diphosphatidylglycerol as well as four glycolipids were identified. 16S rRNA gene sequence analysis revealed that the isolate represents a distinct lineage within the family Coriobacteriaceae and has 94.6 % identity to the type strain of [ Eggerthella ] hongkongensis , the phylogenetically closest bacterial species. On the basis of the analyses performed, the new genus and species Gordonibacter pamelaeae gen. nov., sp. nov. is described, with strain 7-10-1-b T (=DSM 19378 T =CCUG 55131 T ) as the type and only strain of Gordonibacter pamelaeae . Also, based on the chemotaxonomic data obtained for all type strains of the neighbouring genus Eggerthella , we propose that Eggerthella hongkongensis Lau et al. 2006 be transferred to a new genus as Paraeggerthella hongkongensis gen. nov., comb. nov.; the type strain of Paraeggerthella hongkongensis is HKU10 T (=DSM 16106 T =CCUG 49250 T ). Abbreviations: DMA, dimethylacetal The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 7-10-1-b T is AM886059 . A dendrogram derived from biochemical profiles, a maximum-likelihood tree based on 16S rRNA gene sequences, TLC of polar lipids of strain 7-10-1-b T and comparisons of the fatty acid and menaquinone profiles of strain 7-10-1-b T and related type strains are available as supplementary material with the online version of this paper.