Olfactory Representations by Drosophila Mushroom Body Neurons

• Published in: Journal of neurophysiology Vol. 99; no. 2; pp. 734 - 746
• Main Authors: Turner, Glenn C, Bazhenov, Maxim, Laurent, Gilles
• Format: Journal Article
• Language: English
• Published: United States Am Phys Soc 01.02.2008
• Subjects: Anesthetics, Local - pharmacology; Animals; Brain Mapping; Drosophila; Drosophila - anatomy & histology; Electric Stimulation - methods; Excitatory Postsynaptic Potentials - drug effects; Excitatory Postsynaptic Potentials - physiology; Excitatory Postsynaptic Potentials - radiation effects; Membrane Potentials - physiology; Membrane Potentials - radiation effects; Mushroom Bodies - cytology; Nerve Net; Neurons, Afferent - physiology; Odorants; Olfactory Pathways - physiology; Patch-Clamp Techniques - methods; Spectrum Analysis; Tetrodotoxin - pharmacology
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.01283.2007

1 Division of Biology, California Institute of Technology, Pasadena; and 2 The Salk Institute for Biological Studies, La Jolla, California Submitted 27 November 2007; accepted in final form 17 December 2007 Learning and memory has been studied extensively in Drosophila using behavioral, molecular, and genetic approaches. These studies have identified the mushroom body as essential for the formation and retrieval of olfactory memories. We investigated odor responses of the principal neurons of the mushroom body, the Kenyon cells (KCs), in Drosophila using whole cell recordings in vivo. KC responses to odors were highly selective and, thus sparse, compared with those of their direct inputs, the antennal lobe projection neurons (PNs). We examined the mechanisms that might underlie this transformation and identified at least three contributing factors: excitatory synaptic potentials (from PNs) decay rapidly, curtailing temporal integration, PN convergence onto individual KCs is low ( 10 PNs per KC on average), and KC firing thresholds are high. Sparse activity is thought to be useful in structures involved in memory in part because sparseness tends to reduce representation overlaps. By comparing activity patterns evoked by the same odors across olfactory receptor neurons and across KCs, we show that representations of different odors do indeed become less correlated as they progress through the olfactory system. Address for reprint requests and other correspondence: G. Laurent, Div. of Biology, California Institute of Technology, Pasadena, CA 91125 (E-mail: laurentg{at}caltech.edu )