Replacement of recipient stromal/mesenchymal cells after bone marrow transplantation using bone fragments and cultured osteoblast-like cells

• Published in: Biology of blood and marrow transplantation Vol. 10; no. 10; pp. 709 - 717
• Main Authors: Cahill, Richard A., Jones, Olcay Y., Klemperer, Martin, Steele, Anne, Mueller, Thomas O., El-Badri, Nagwa, Chang, Y., Good, Robert A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2004
• Subjects: Bone fragments; Bone Marrow Transplantation - methods; Bone Transplantation; Cells, Cultured; Child; Female; Graft Survival; Humans; Infant; Male; Mesenchymal cells; Mesenchymal Stromal Cells - cytology; Osteoblasts; Osteoblasts - cytology; Osteoblasts - transplantation; Pilot Projects; Regeneration; Stroma; Stromal Cells - cytology; Transplantation, Homologous; Treatment Outcome; Stroma; Mesenchymal cells; Bone fragments; Osteoblasts
• ISSN: 1083-8791; 1523-6536
• DOI: 10.1016/j.bbmt.2004.06.001

We present our experience on treatment of three children with potentially fatal diseases using a unique protocol for non-myeloablative bone marrow transplantation. The protocol was designed to promote engraftment of bone marrow stromal/mesenchymal cells (SC/MSCs) based on the knowledge from preclinical models over the last three decades. Accordingly, our protocol is the first to test the use of bone fragments as an ideal vehicle to transplant such cells residing in the bone core. Because of the paucity of knowledge for optimum transplantation of SC/MSCs in humans, we used a multifaceted approach and implanted bone fragments both intraperitoneally and directly into bone on day 0 of BMT. We also infused cultured donor osteoblast-like cells intravenously post-BMT. We were able to achieve high levels of stroma cell engraftment as defined by molecular analyses of bone biopsy specimens.