The genetic polymorphism of merozoite surface protein-1 in Plasmodium falciparum isolates from Aceh province, Indonesia

• Published in: IOP conference series. Earth and environmental science Vol. 125; no. 1; pp. 12037 - 12042
• Main Authors: Jamil, K F, Supargiyono, S, Syafruddin, D, Pratama, N, Silvy, S
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.03.2018
• Subjects: Alleles; Erythrocytes; Gene polymorphism; Genetic diversity; Laboratory tests; Malaria; Mixed infection; Polymorphism; Vector-borne diseases; Indonesia
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/125/1/012037

An estimated of 3.3 million Indonesian population were infected with malaria. However, extensive genetic polymorphism of the field isolates msp-1 of P. falciparum represents a major obstacle for the development of malaria treatment. The aim of this study was to investigate the genetic diversity of msp-1 genotype in field isolates of P. falciparum collected in Aceh Province. A total of 90 patients with malaria (+) were selected from eleven district hospitals in Aceh from 2013-2015. Data were collected by anamnesis, complete physical examination and laboratory tests for msp-1. All protocols to diagnose malaria followed the WHO 2010 guideline. All samples were stored in Eijkman Biology Molecular Institute, Jakarta. Among 90 samples, 57.7% were male, and 42.3% were female with the most cases found between 21-30 years old. From the allele typing analysis of P. falciparum from Aceh; K1, MAD20, and RO33 allele types were identified. MAD20 type was the highest allele found in this study (57.9%). It was found in single and mixed infection. A moderate level of the mixed allele was also observed.