Development of a QuEChERS method for simultaneous analysis of 3-Monochloropropane-1,2-diol monoesters and Glycidyl esters in edible oils and margarine by LC-APCI-MS/MS

• Published in: Analytica chimica acta Vol. 1239; p. 340712
• Main Authors: Custodio-Mendoza, Jorge A., Sendón, Raquel, de Quirós, Ana Rodríguez-Bernaldo, Lorenzo, Rosa A., Carro, Antonia M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.01.2023
• Subjects: 3-MCPD; alpha-Chlorohydrin - analysis; APCI; Esters - analysis; Food contaminants; Food Contamination - analysis; Glycidyl; HPLC method; Margarine - analysis; Oleic Acid - analysis; Olive Oil - analysis; Plant Oils - analysis; QuEChERS; Tandem Mass Spectrometry - methods; HPLC method; 3-MCPD; APCI; Food contaminants; Glycidyl; QuEChERS
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2022.340712

A simple, fast and effective direct method based on HPLC-APCI-QqQ-MS/MS has been developed to simultaneously determine four 3-monochloropropane-1,2-diol monoesters (3-MCPDE) esterified with palmitic, linoleic, stearic, and oleic acid, and two glycidyl esters (GE) with palmitic and oleic acid in margarine and olive oil using a QuEChERS approach. Factors affecting the efficiency of the extraction process were assessed, including type and amount of salt, extraction solvent, test portion amount, and clean-up sorbent. The analytical method was validated according to Food and Drug Administration (FDA) guidelines using matrix-matched calibration with internal standards and showed good results in terms of linearity (r2 > 0.9992), accuracy (80<Recovery<120%), and precision (RSD<15%). The method was successfully applied for the first time to 11 margarine samples for simultaneous analysis of 3-MCPDE and GE. [Display omitted] •Direct determination using atmospheric-pressure chemical ionization (APCI) source.•Comparison of APCI and HESI-II sources in the analysis of 3-MCPDE and GE.•Cost-effective approach with simpler mobile phases and no matrix effect.•Novel QuEChERS approach to extract of 3-MCPDE and GE from margarines.•The presence of glycidyl oleate was confirmed in all analyzed samples.