Microbial biotransformation of N-nitro-, C-nitro-, and C-nitrous-type mutagens by Lactobacillus delbrueckii subsp. bulgaricus in meat products

Processed meats are classified by the International Agency for Research on Cancer (IARC) as carcinogenic to humans. However, information on the responsible agents and the influence of industrial processing on the increased risk of cancer is still lacking. This study aimed to use cultures of Lactobac...

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Bibliographic Details
Published inFood and chemical toxicology Vol. 136; p. 110964
Main Authors Molognoni, Luciano, Motta, Gabriel Emiliano, Daguer, Heitor, De Dea Lindner, Juliano
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.02.2020
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Summary:Processed meats are classified by the International Agency for Research on Cancer (IARC) as carcinogenic to humans. However, information on the responsible agents and the influence of industrial processing on the increased risk of cancer is still lacking. This study aimed to use cultures of Lactobacillus delbrueckii subsp. bulgaricus LB-UFSC 01 to biodegrade harmful C-nitrous, N-nitro, and C-nitro compounds in processed meat matrix. Firstly, positive results for ethylnitrolic acid (ENA) (>5.00 μg kg−1) and 2-methyl-1,4-dinitro-pyrrole (DNMP) (>12.0 μg kg−1) were obtained in mortadellas produced under different experimental conditions employing preservatives and antioxidants. Mortadellas containing nitrite and sorbate in the ratio of 8:1 (w/w) yielded the highest concentrations of mutagens. However, the treatment with the LB-UFSC 01 culture was able to modulate the harmful compounds in the mortadella samples. Several analytical methods employing liquid chromatography coupled to mass spectrometry and statistical models were employed to identify the metabolites and reaction routes during microbial biotransformation. For the first time, relevant information regarding the formation and degradation of ENA and DNMP in a processed meat model simulating real conditions was presented. •ENA and DNMP mutagenic products were identified in a mortadella model.•Lb. bulgaricus modulated noxious C-nitrous, N-nitro &C-nitro compounds in mortadella.•Main routes of ENA and DNMP bioconversion by Lb. bulgaricus were presented.•Lb. bulgaricus used enzymes to eliminate the potential mutagenic of the compounds.•Several LC-MS methods and statistical models were employed in the study.
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2019.110964