Comparative Whole Metagenome Analysis in Lesional and Nonlesional Scalp Areas of Patients with Psoriasis Capitis and Healthy Individuals

• Published in: Journal of investigative dermatology Vol. 145; no. 3; pp. 605 - 617.e14
• Main Authors: De Pessemier, Britta, López, Celia Díez, Taelman, Steff, Verdonck, Merel, Chen, Yang, Stockman, Annelies, Lambert, Jo, Van de Wiele, Tom, Callewaert, Chris
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2025
• Subjects: Adult; Case-Control Studies; Female; Humans; Male; Metagenome; Metagenomics; Microbiota - genetics; Middle Aged; Pathogenomics; Psoriasis - microbiology; Psoriasis - pathology; Psoriasis capitis; RNA, Ribosomal, 16S - genetics; Scalp - microbiology; Scalp - pathology; Scalp microbiome; Skin microbiome; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; VF; Metagenomics; Pathogenomics; Scalp microbiome; AMP; FDR; AMR; rRNA; RND; Psoriasis capitis; Skin microbiome
• ISSN: 0022-202X; 1523-1747; 1523-1747
• DOI: 10.1016/j.jid.2024.07.020

Psoriasis is an immune-mediated inflammatory disorder, where the majority of the patients suffer from psoriasis capitis or scalp psoriasis. Current therapeutics remain ineffective to treat scalp lesions. In this study, we present a whole-metagenome characterization of the scalp microbiome in psoriasis capitis. We investigated how changes in the homeostatic cutaneous microbiome correlate with the condition and identified metagenomic biomarkers (taxonomic, functional, virulence factors, antimicrobial resistance genes) that could partly explain its emergence. Within this study, 83 top and back scalp samples from healthy individuals and 64 lesional and nonlesional scalp samples from subjects with untreated psoriasis capitis were analyzed. Using qPCR targeting the 16S and 18S ribosomal RNA genes, we found a significant decrease in microbial load within scalp regions affected by psoriasis compared with that in their nonlesional counterparts. Metagenomic analysis revealed that psoriatic lesions displayed significant lower Cutibacterium species (including C. modestum, C. namnetense, C. granulosum, C. porci), along with an elevation in Staphylococcus aureus. A heightened relative presence of efflux pump protein–encoding genes was detected, suggesting potential antimicrobial resistance mechanisms. These mechanisms are known to specifically target human antimicrobial peptides (including cathelicidin LL-37), which are frequently encountered within psoriasis lesions. These shifts in microbial community dynamics may contribute to psoriasis disease pathogenesis.