Expressional and functional studies of CKLF1 during dendritic cell maturation

• Published in: Cellular immunology Vol. 263; no. 2; pp. 188 - 195
• Main Authors: Shao, Luning, Li, Ting, Mo, Xiaoning, Majdic, Otto, Zhang, Yanfei, Seyerl, Maria, Schrauf, Catharina, Ma, Dalong, Stöckl, Johannes, Han, Wenling
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 2010
• Subjects: Antigen-presenting cells; Cell Differentiation - drug effects; Chemokine-like factor 1; Chemokines - metabolism; Chemokines - pharmacology; Dendritic cell; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - immunology; Down-Regulation; Gene Expression Regulation; HLA-DR; Humans; IFN-γ; IL-12; Interleukin-12 - secretion; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - immunology; MARVEL Domain-Containing Proteins; Dendritic cell; IFN-γ; HLA-DR; IL-12; Chemokine-like factor 1
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/j.cellimm.2010.03.015

Chemokine-like factor 1 (CKLF1) was the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family to be discovered. Its expression level is increased clearly in peripheral blood lymphocytes upon phytohemagglutinin stimulation, but little is known about the expression and function of CKLF1 in dendritic cells (DCs), which are the most potent antigen-presenting cells. In the present study, we showed that CKLF1 was highly expressed in monocytes. During differentiation from monocytes to immature DCs, CKLF1 was increased dramatically on day 2 and then decreased from day 3 to 5. Upon maturation with different stimuli, CKLF1 was down-regulated. Two peptides of CKLF1, C19 and C27, stimulated the effect of immature DCs (imDCs) on T-cell proliferation and IFN-γ production. Further study on DC maturation showed that C19 and C27 up-regulated HLA-DR expression and IL-12 secretion, with no obvious effects on CD80, CD83 or CD86. Thus, CKLF1-C19 and -C27 can stimulate antigen-presenting capability of imDCs.