A novel 78-kDa fatty acyl-CoA synthetase (ACS1) of Babesia bovis stimulates memory CD4 + T lymphocyte responses in B. bovis-immune cattle
Antigen-specific CD4 + T lymphocyte responses contribute to protective immunity against Babesia bovis, however the antigens that induce these responses remain largely unknown. A proteomic approach was used to identify novel B. bovis antigens recognized by memory CD4 + T cells from immune cattle. Fra...
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Published in | Molecular and biochemical parasitology Vol. 147; no. 1; pp. 20 - 29 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.05.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Antigen-specific CD4
+ T lymphocyte responses contribute to protective immunity against
Babesia bovis, however the antigens that induce these responses remain largely unknown. A proteomic approach was used to identify novel
B. bovis antigens recognized by memory CD4
+ T cells from immune cattle. Fractions obtained from merozoites separated by continuous-flow electrophoresis (CFE) that contained proteins ranging from 20 to 83
kDa were previously shown to stimulate memory CD4
+ lymphocyte responses in
B. bovis-immune cattle. Expression library screening with rabbit antiserum raised against an immunostimulatory CFE fraction identified a clone encoding a predicted 78
kDa protein. BLAST analysis revealed sequence identity of this
B. bovis protein with
Plasmodium falciparum fatty acyl coenzyme A synthetase (ACS) family members (PfACS1–PfACS11), and the protein was designated
B. bovis acyl-CoA synthetase 1 (ACS1). Southern blot analysis indicated that
B. bovis ACS1 is encoded by a single gene, although BLAST analysis of the preliminary
B. bovis genome sequence identified two additional family members, ACS2 and ACS3. Peripheral blood lymphocytes and CD4
+ T cell lines from
B. bovis-immune cattle proliferated significantly against recombinant ACS1 protein, consistent with its predicted involvement in protective immunity. However, immune sera from cattle recovered from
B. bovis infection did not react with ACS1, indicating that epitopes may be conformationally dependent. |
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Bibliography: | http://dx.doi.org/10.1016/j.molbiopara.2006.01.004 http://hdl.handle.net/10113/48961 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0166-6851 1872-9428 |
DOI: | 10.1016/j.molbiopara.2006.01.004 |