Exploration of glycosyltransferases mutation status in cervical cancer reveals PARP14 as a potential prognostic marker

• Published in: Glycoconjugate journal Vol. 40; no. 5; pp. 513 - 522
• Main Authors: Wang, Hui, Luo, Shen, Wu, Xin, Ruan, Yuanyuan, Qiu, Ling, Feng, Hao, Zhu, Shurong, You, Yanan, Li, Ming, Yang, Wenting, Zhao, Yanding, Tao, Xiang, Jiang, Hua
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2023; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Cervical cancer; Diagnosis; Gene expression; Glycosylation; Immunohistochemistry; Life Sciences; Medical diagnosis; Medical prognosis; Mutation; Pathology; Patients; Survival analysis; Tumors; PARP14 (poly (ADP-ribose) polymerase member 14); Mutation; Cervical Cancer; Glycosyltransferase (GT)
• ISSN: 0282-0080; 1573-4986; 1573-4986
• DOI: 10.1007/s10719-023-10134-7

This study investigates the potential role of Glycosyltransferases (GTs) in the glycosylation process and their association with malignant tumors. Specifically, the study focuses on PARP14, a member of GTs, and its potential as a target for tumors in the diagnosis and treatment of cervical cancer. To gather data, the study used somatic mutation data, gene expression data and clinical information from TCGA-CESE dataset as well as tissue samples from cervical cancer patients. Further verification was conducted through RT-qPCR and immunohistochemistry staining on cervical cancer tissues to confirm the expression of PARP14. The study utilized Kaplan-Meier for survival analysis of cervical cancer patient and found significant mutational abnormalities in GTs. The high frequency mutated gene was identified as PARP14. RT-qPCR revealed significantly higher mRNA expression of PARP14 compared to precancerous tissue. Using IHC combined with Kaplan-Meier,patients in the PARP14 high expression group had a better prognosis than the low expression group. The study identified PARP14 as a frequently mutated gene in cervical cancer and proposed its potential role in diagnosis and treatment.