Assaying benzene, a parquet varnish, and a synthetic thinner with respect to induction of in vivo chromosome loss in wing primordial cells of Drosophila

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 763; pp. 18 - 22
• Main Authors: Soós, István, Szabad, János
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2014; Elsevier BV
• Subjects: Animals; Animals, Genetically Modified; Benzene; Benzene - chemistry; Benzene - toxicity; Cells; Chromosome Deletion; Chromosome loss; Chromosomes; Cloning; Drosophila; Drosophila - drug effects; Drosophila - genetics; Drosophila - metabolism; Drosophila Proteins - genetics; Genetic engineering; Germ Cells - drug effects; Germ Cells - metabolism; Germ Cells - ultrastructure; Hydrocarbons; Insects; Paint - toxicity; Parquet varnish; Polyurethanes - chemistry; Polyurethanes - toxicity; Solvents - chemistry; Solvents - toxicity; Synthetic thinner; Volatilization; Wing clones; Wings, Animal - drug effects; Wings, Animal - embryology; Wings, Animal - ultrastructure; Y Chromosome - genetics; Synthetic thinner; Wing clones; Benzene; Drosophila; Chromosome loss; Parquet varnish
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/j.mrgentox.2013.11.007

•In vivo loss of a Y chromosome leads to clone formation in the Drosophila wings.•Strength of Y loss can be quantified based frequency and size of the clones.•We confirm and measure the aneugenic activity of benzene.•Vapors of a parquet varnish and a synthetic thinner induce chromosome loss. An assay detecting the in vivo loss of mwh+Y, a genetically engineered Y chromosome, in cells of the Drosophila wing primordia was published recently. Loss of the mwh+Y chromosome in any of the wing-disk cells – in a multiple wing hairs homozygous background – leads to the formation of an mwh mosaic spot (clone) in the emerging wing. The frequency and the size of the mwh clones allow detection and quantitative evaluation of environmental and/or genetic agents inducing chromosome loss. Using this novel technique, we analyzed the potential of vapors of benzene, a parquet varnish, and a synthetic thinner to induce chromosome loss. Exposure to 0.047μg/ml benzene vapor for one day or to 0.175μg/ml for four hours resulted in a significantly elevated mwh clone-frequency confirming the ability of benzene to induce chromosome loss. A one-day exposure to vapors of a parquet varnish or a 6-h exposure to vapors of a synthetic thinner slightly, yet significantly elevated the frequency of chromosome loss. Results of the present paper show the potential of vapors of the analyzed parquet varnish and synthetic thinner to induce chromosome loss, and illustrate the usefulness of the new technique.