cfDNA in exhaled breath condensate (EBC) and contamination by ambient air: toward volatile biopsies

Exhaled breath is a source of volatile and nonvolatile biomarkers in the body that can be accessed non-invasively and used for monitoring. The collection of lung secretions by conventional methods such as bronchoalveolar lavage, induced sputum collection, and core biopsies is limited by the invasive...

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Published inJournal of breath research Vol. 13; no. 3; pp. 36006 - 36010
Main Authors Koc, Altug, Goksel, Tuncay, Pelit, Levent, Korba, Korcan, Dizdas, Tugberk N, Baysal, Ertan, Uzun, Umut C, Kaya, Ozge Ozer, Ozyilmaz, Berk, Kutbay, Yasar B, Ozdemir, Taha Resid, Kirbiyik, Ozgur, Erdogan, Kadri M, Guvenc, Merve Saka, Kocal, Gizem Calibasi, Basbinar, Yasemin
Format Journal Article
LanguageEnglish
Published England IOP Publishing 01.05.2019
Subjects
Adult
Air - analysis
Biomarkers
Biopsy
Breath Tests - methods
Cell-Free Nucleic Acids - analysis
cfDNA
DNA Contamination
Exhalation
exhaled breath condensate
Female
Gene Dosage
Genes, Essential
Humans
Lung cancer
Male
Middle Aged
volatile condensate
Volatilization
Young Adult
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Summary:Exhaled breath is a source of volatile and nonvolatile biomarkers in the body that can be accessed non-invasively and used for monitoring. The collection of lung secretions by conventional methods such as bronchoalveolar lavage, induced sputum collection, and core biopsies is limited by the invasive nature of these methods. Non-invasive collection of exhaled breath condensate (EBC) provides fluid samples that are representative of airway lining fluids. Various volatile and nonvolatile biomarkers can be detected in volatile condensates, such as H2O2, nitric oxide, lipid mediators, cytokines, chemokines, DNA, and microRNAs. Studies have examined cell-free DNA (cfDNA) in plasma samples from non-small-cell lung cancer patients, offering to new insights and fostering development of the liquid biopsy. However, few studies have examined cfDNA in EBC samples. This study examined whether EBC is an appropriate source of cfDNA using housekeeping-gene-specific primer probes and quantitative real-time polymerase chain reaction in healthy subjects. Ambient (room) air is contaminated with DNA, so caution is needed. Preliminary studies indicated that volatile biopsies are becoming an important diagnostic tool in lung cancer.
Bibliography:JBR-100941.R1
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ISSN:1752-7163
1752-7155
1752-7163
DOI:10.1088/1752-7163/ab17ff