Negative prognostic impact of regulatory T cell infiltration in surgically resected esophageal cancer post-radiochemotherapy

• Published in: Oncotarget Vol. 6; no. 25; pp. 20840 - 20850
• Main Authors: Vacchelli, Erika, Semeraro, Michaela, Enot, David P, Chaba, Kariman, Poirier Colame, Vichnou, Dartigues, Peggy, Perier, Aurelie, Villa, Irene, Rusakiewicz, Sylvie, Gronnier, Caroline, Goéré, Diane, Mariette, Christophe, Zitvogel, Laurence, Kroemer, Guido
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 28.08.2015
• Subjects: Alleles; Apoptosis; CD8-Positive T-Lymphocytes - cytology; Chemoradiotherapy - methods; Cohort Studies; Esophageal Neoplasms - drug therapy; Esophageal Neoplasms - immunology; Esophageal Neoplasms - radiotherapy; Female; Forkhead Transcription Factors - metabolism; Gene Expression Regulation, Neoplastic; Genotype; Humans; Immunohistochemistry; Lymphocytes, Tumor-Infiltrating - immunology; Male; Priority Research Paper; Prognosis; Receptors, Purinergic P2X7 - metabolism; T-Lymphocytes, Regulatory - cytology; Toll-Like Receptor 4 - metabolism; Treatment Outcome; apoptosis; autophagy; ATG16L1; pattern recognition receptor; immunogenic cell death
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.4428

Ever accumulating evidence indicates that the long-term effects of radiotherapy and chemotherapy largely depend on the induction (or restoration) of an anticancer immune response. Here, we investigated this paradigm in the context of esophageal carcinomas treated by neo-adjuvant radiochemotherapy, in a cohort encompassing 196 patients. We found that the density of the FOXP3+ regulatory T cell (Treg) infiltrate present in the residual tumor (or its scar) correlated with the pathological response (the less Tregs the more pronounced was the histological response) and predicted cancer-specific survival. In contrast, there was no significant clinical impact of the frequency of CD8+ cytotoxic T cells. At difference with breast or colorectal cancer, a loss-of-function allele of toll like receptor 4 (TLR4) improved cancer-specific survival of patients with esophageal cancer. While a loss-of-function allele of purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7) failed to affect cancer-specific survival, its presence did correlate with an increase in Treg infiltration. Altogether, these results corroborate the notion that the immunosurveillance seals the fate of patients with esophageal carcinomas treated with conventional radiochemotherapy.