Chronic intrahippocampal interleukin-1β overexpression in adolescence impairs hippocampal neurogenesis but not neurogenesis-associated cognition

• Published in: Brain, behavior, and immunity Vol. 83; pp. 172 - 179
• Main Authors: Pawley, Lauren C., Hueston, Cara M., O'Leary, James D., Kozareva, Danka A., Cryan, John F., O'Leary, Olivia F., Nolan, Yvonne M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.01.2020
• Subjects: Adolescence; Aging - metabolism; Animals; Behavior; Cognition; Hippocampus; Hippocampus - cytology; Hippocampus - metabolism; IL-1β; Inflammation; Interleukin-1beta - metabolism; Male; Neurogenesis; Rats; Rats, Sprague-Dawley; Adolescence; Inflammation; Behavior; IL-1β; Neurogenesis; Hippocampus
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2019.10.007

•IL-1β overexpression in adolescent rat dorsal hippocampus reduced hippocampal neurogenesis.•IL-1β overexpression in adolescent rat dorsal hippocampus reduced neurite branching.•IL-1β overexpression in adolescent rat dorsal hippocampus did not affect cognition. Both neuroinflammation and adult hippocampal neurogenesis (AHN) are implicated in many neurodegenerative disorders as well as in neuropsychiatric disorders, which often become symptomatic during adolescence. A better knowledge of the impact that chronic neuroinflammation has on the hippocampus during the adolescent period could lead to the discovery of new therapeutics for some of these disorders. The hippocampus is particularly vulnerable to altered concentrations of the pro-inflammatory cytokine interleukin-1β (IL-1β), with elevated levels implicated in the aetiology of neurodegenerative disorders such as Alzheimer’s and Parkinson’s, and stress-related disorders such as depression. The effect of acutely and chronically elevated concentrations of hippocampal IL-1β have been shown to reduce AHN in adult rodents. However, the effect of exposure to chronic overexpression of hippocampal IL-1β during adolescence, a time of increased vulnerability, hasn’t been fully interrogated. Thus, in this study we utilized a lentiviral approach to induce chronic overexpression of IL-1β in the dorsal hippocampus of adolescent male Sprague Dawley rats for 5 weeks, during which time its impact on cognition and hippocampal neurogenesis were examined. A reduction in hippocampal neurogenesis was observed along with a reduced level of neurite branching on hippocampal neurons. However, there was no effect of IL-1β overexpression on performance in pattern separation, novel object recognition or spontaneous alternation in the Y maze. Our study has highlighted that chronic IL-1β overexpression in the hippocampus during the adolescent period exerts a negative impact on neurogenesis independent of cognitive performance, and suggests a degree of resilience of the adolescent hippocampus to inflammatory insult.