Method Validation for Simultaneous Determination of Cholesterol and Cholesterol Oxides in Milk by RP-HPLC-DAD

The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification an...

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Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 25; no. 1; pp. 161 - 168
Main Authors Bauer, Luciana C., Santana, Débora de A., Macedo, Marianne dos S., Torres, Alexandre G., Souza, Nilson E. de, Simionato, Julliana I.
Format Journal Article
LanguageEnglish
Portuguese
Published Sociedade Brasileira de Química 01.01.2014
Subjects
CHEMISTRY, MULTIDISCIPLINARY
25-hydroxycholesterol
dairy fat
liquid chromatography
direct saponification
7-ketocholesterol
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Summary:The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.
ISSN:0103-5053
1678-4790
DOI:10.5935/0103-5053.20130283