Detection of Fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay

• Published in: Parasitology research (1987) Vol. 104; no. 1; pp. 155 - 161
• Main Authors: Kumar, Niranjan, Ghosh, S, Gupta, S. C
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.12.2008; Springer-Verlag; Springer
• Subjects: Animals; Antibodies, Helminth - blood; Antigens, Helminth - immunology; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Buffaloes - immunology; Buffaloes - parasitology; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - immunology; Cattle Diseases - parasitology; Enzyme-Linked Immunosorbent Assay - veterinary; Fasciola - immunology; Fascioliasis - diagnosis; Fascioliasis - parasitology; Fascioliasis - veterinary; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Glycoproteins - immunology; Immunology; Invertebrates; Medical Microbiology; Microbiology; Original Paper; Sensitivity and Specificity; Indian Veterinary Research Institute; Strip Number; Serum Number; Seron Sample; Somatic Antigen; Plathelmintha; Parasite; Fasciola gigantica; Trematoda; Infection; Vertebrata; Mammalia; Helmintha; Artiodactyla; Invertebrata; Detection; ELISA assay; Ungulata; Buffalo
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-008-1174-2

The process of isolation of the 27-kDa glycoprotein from the somatic antigen of Fasciola gigantica was standardized and the diagnostic potentiality was evaluated for the detection of bubaline fasciolosis by indirect enzyme-linked immunosorbent assay. Initially, the test was standardized using the sera from experimentally noninfected(n = 20) and infected (n = 5)animals. Further, the sensitivity and the specificity of the test were evaluated through the sera of buffaloes with different natural infections, i.e., F. gigantica (n = 8 animals), F. gigantica and Gastrothylax crumenifer(n = 15), F. gigantica and Gigantocotyle explanatum (n = 6), trematode infections other than F. gigantica (n = 9), only G. crumenifer (n = 36), only G. explanatum (n = 18), G. crumenifer and G. explanatum positive (n = 39), and PM negative (n = 102). All animals came from the slaughterhouses of Bareilly (Uttar Pradesh, India) and Patna (Bihar, India). The level of sensitivity observed in the present study was 81.0%, while 97-98% specificity against G. crumenifer, G. explanatum, or a mixed infection with both parasites was noted. The study showed F. gigantica prevalence rate of 18-20% in the buffaloes of the study area. Enzyme-linked immunosorbent assay with a 27-kDa glycoprotein could be a feasible diagnostic method for the early detection of bovine fasciolosis.