Genetic Diversity of Brucella Reference and Non-reference Phages and Its Impact on Brucella -Typing

• Published in: Frontiers in microbiology Vol. 8; p. 408
• Main Authors: Hammerl, Jens A, Göllner, Cornelia, Jäckel, Claudia, Scholz, Holger C, Nöckler, Karsten, Reetz, Jochen, Al Dahouk, Sascha, Hertwig, Stefan
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 15.03.2017
• Subjects: Microbiology; genome; virulent; phage; lysotyping; Brucella
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2017.00408

Virulent phages have been used for many years to type isolates, but until recently knowledge about the genetic makeup of these phages remains limited. In this work the host specificity and genomic sequences of the original set (deposited in 1960) of VLA reference phages Tb, Fi, Wb, Bk2, R/C, and Iz were analyzed and compared with hitherto described brucellaphages. VLA phages turned out to be different from homonymous phages in other laboratories. The host range of the phages was defined by performing plaque assays with a wide selection of strains. Propagation of the phages on different strains did not alter host specificity. Sequencing of the phages Tb , Fi , Wb , and R/C revealed nucleotide variations when compared to same-named phages previously described by other laboratories. The phages Bk2 and Iz were sequenced for the first time. While Bk2 exhibited the same deletions as Wb , Iz possesses the largest genome of all reference phages. The duplication of a 301 bp sequence in this phage and the large deletion in Bk2 , Wb , and R/C may be a result of recombination caused by repetitive sequences located in this DNA region. To identify new phages as potential candidates for lysotyping, the host range and Single Nucleotide Polymorphisms (SNPs) of 22 non-reference phages were determined. The phages showed lysis patterns different from those of the reference phages and thus represent novel valuable candidates in the typing set.