Cytotoxicity responses to selected ESAT-6 and CFP-10 peptides in tuberculosis

Cytotoxicity responses were studied for the ESAT-6 peptides Esp1, Esp6, Esp7, Esp8, and CFP-10 peptides, Cfp6, Cfp7, Cfp8, Cfp9 (synthetic 20-mer peptides) and the recombinant ESAT-6, CFP-10 proteins. Cytolytic molecules perforin, granzymes A and B, granulysin responses in healthy household contacts...

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Bibliographic Details
Published inCellular immunology Vol. 265; no. 2; pp. 146 - 155
Main Authors Madhan Kumar, M., Raja, Alamelu
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 2010
Subjects
Adult
Antigens, Bacterial - chemistry
Antigens, Bacterial - immunology
Bacterial Proteins - chemistry
Bacterial Proteins - immunology
CD4 antigen
CD4-Positive T-Lymphocytes - enzymology
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - enzymology
CD8-Positive T-Lymphocytes - immunology
CFP-10
Cytotoxicity
Cytotoxicity, Immunologic
ESAT-6
Family
Female
Granulysin
Granzyme
Granzymes - metabolism
Humans
Lymphocyte Activation
Male
Middle Aged
Mycobacterium
Mycobacterium tuberculosis - immunology
Peptides - chemistry
Peptides - immunology
Perforin
Recombinant Proteins - chemistry
Recombinant Proteins - immunology
Tuberculosis, Pulmonary - immunology
Tuberculosis, Pulmonary - prevention & control
Tuberculosis, Pulmonary - transmission
Granzyme
Cytotoxicity
CFP-10
ESAT-6
Perforin
Granulysin
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Summary:Cytotoxicity responses were studied for the ESAT-6 peptides Esp1, Esp6, Esp7, Esp8, and CFP-10 peptides, Cfp6, Cfp7, Cfp8, Cfp9 (synthetic 20-mer peptides) and the recombinant ESAT-6, CFP-10 proteins. Cytolytic molecules perforin, granzymes A and B, granulysin responses in healthy household contacts (HHC) and pulmonary tuberculosis patients (PTB), were studied by intracellular flow cytometry. Functional cytotoxicity was studied in both the groups for the peptides Esp6 and Cfp8 by an enzyme (lactate dehydrogenase) based assay. The results revealed that cytolytic molecule positive CD4+ and CD8+ T cells were increased in HHC in response to Esp1, Esp6, Cfp8 and Cfp9 immunogenic peptides compared to PTB. Functional cytotoxicity results showed higher cytotoxicity (not statistically significant) to be exhibited by the peptide Esp6 than Cfp8 in the HHC.
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ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2010.08.004