Exercise intensity and lymphocyte subset apoptosis

• Published in: International journal of sports medicine Vol. 34; no. 3; p. 268
• Main Authors: Navalta, J W, Lyons, S, Prestes, J, Arnett, S W, Schafer, M, Sobrero, G L
• Format: Journal Article
• Language: English
• Published: Germany 01.03.2013
• Subjects: Adult; Antigens, CD19; Apoptosis - physiology; B-Lymphocyte Subsets - physiology; CD4-Positive T-Lymphocytes - physiology; CD8-Positive T-Lymphocytes - physiology; Exercise - physiology; Exercise Test; Humans; Male; Physical Endurance; T-Lymphocyte Subsets - physiology; Transendothelial and Transepithelial Migration - physiology
• ISSN: 1439-3964
• DOI: 10.1055/s-0032-1312581

This investigation assessed the lymphocyte subset response to increasing intensity. Participants completed an exertion test (VO(2max)), and later performed a 10-min run at 76% VO(2max), 5-min at 87%, and run to exhaustion at 100% intensity. Blood was sampled at rest, following each intensity, and 1-h post. Cell concentration, apoptosis (annexin V) and migration (CX₃CR1) were evaluated in CD4+, CD8+, and CD19+ subsets. Relative data were analyzed using 1-way ANOVA with significance at P≤0.05. Absolute changes from rest (Δ baseline) were calculated for exercise conditions. CX₃CR1 displayed relative changes 1-h post, (CD8+ Pre=58%, Post=68%, 1 h-Post=37%, P=0.04) (CD19+ Pre=1.9%, Post=3.2%, 1 h-Post=5.2%, P=0.02). No relative changes were noted for subsets and annexin V. Absolute changes revealed that CD4+/annexin V+ and CD8+/annexin V+ significantly increased at 76%,(P<0.01). Significant absolute increases were observed in CD4+/CX₃CR1 at 87% VO2max, and at 87% and 100% VO2max in CD8+/CX₃CR1 (P<0.01). Subsets respond differently with intensity with respect to cell count, and markers of apoptosis and cell migration. CD4+ and CD8+ appear to be prone to apoptosis with moderate exercise, but significant increases in migration at higher intensities suggests movement of these cells from the vasculature in postexercise measurements.