Evaluation of the DiversiLab® automated repetitive sequence-based PCR system for the characterization of Candida albicans and Candida glabrata isolates

• Published in: Journal de mycologie médicale Vol. 28; no. 2; pp. 320 - 326
• Main Authors: Sasso, M., Normand, A.-C., Pantel, A., Bourgeois, N., Lachaud, L.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.06.2018; Elsevier Masson
• Subjects: Automation; Candida albicans - classification; Candida albicans - isolation & purification; Candida glabrata - classification; Candida glabrata - isolation & purification; Candida albicans; Candida glabrata; Candidiasis - diagnosis; Candidiasis - microbiology; DiversiLab; Genotype; Humans; Life Sciences; Microsatellite Repeats; MultiLocus microsatellite typing; MultiLocus sequence typing; Mycological Typing Techniques; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - standards; Rep-PCR; Repetitive Sequences, Nucleic Acid; Reproducibility of Results; Typing method; Candida glabrata; Rep-PCR; Candida albicans; DiversiLab; Typing method; MultiLocus sequence typing; MultiLocus microsatellite typing; DiversiLab(®); Candida albicans; Reproducibility of Results; Automation; Candidiasis; Candida~albicans; Repetitive Sequences; Humans; DiversiLab(\textregistered); Genotype; Candida glabrata; Nucleic Acid; Candida~glabrata; Polymerase Chain Reaction; Microsatellite Repeats; Mycological Typing Techniques
• ISSN: 1156-5233; 1773-0449
• DOI: 10.1016/j.mycmed.2018.03.003

The objective of our study was to assess the DiversiLab® automated repetitive sequence-based PCR (rep-PCR) system for typing C. albicans and C. glabrata isolates and to compare it with two proven and routinely used typing methods. A total of 39 isolates from 11 patients with candidaemia or tissue candidiasis (two to six isolates per patient) were analyzed with three typing methods: DiversiLab® rep-PCR, multilocus sequence typing and multilocus microsatellite typing. DiversiLab® rep-PCR results were consistent with those obtained using the two other typing methods for C. albicans, but not for C. glabrata. Thanks to its simplicity of use, rapidity, standardization and reproducibility, the DiversiLab® rep-PCR system is an interesting tool to investigate C. albicans infections.