Huangqi Baihe Granules alleviate hypobaric hypoxia-induced acute lung injury in rats by suppressing oxidative stress and the TLR4/NF-κB/NLRP3 inflammatory pathway

• Published in: Journal of ethnopharmacology Vol. 324; p. 117765
• Main Authors: Zeng, Yuanding, Cao, Wangjie, Huang, Yong, Zhang, Han, Li, Congyi, He, Jianzheng, Liu, Yongqi, Gong, Hongxia, Su, Yun
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 24.04.2024
• Subjects: Acute lung injury; Acute Lung Injury - chemically induced; Animals; Astragalus propinquus; Cytokines - metabolism; Drugs, Chinese Herbal; Glutathione - metabolism; Huangqi Baihe Granule; Hypobaric hypoxia; Hypoxia - complications; Hypoxia - drug therapy; Inflammation; Lipopolysaccharides - pharmacology; NF-kappa B - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Oxidative Stress; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism; Toll-Like Receptor 4 - metabolism; Tumor Necrosis Factor-alpha - metabolism; Hypobaric hypoxia; Huangqi Baihe Granule; Acute lung injury; Oxidative stress; Inflammation
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2024.117765

Huangqi Baihe Granules (HQBHG) are a modified formulation based on the traditional recipe “Huangqi Baihe porridge” and the Dunhuang medical prescription “Cistanche Cistanche Soup.” The Herbal medicine moistens the lungs and tones the kidneys in addition to replenishing Qi and feeding Yin, making it an ideal choice for enhancing adaptability to high-altitude hypoxic environments. The purpose of this study was to examine a potential molecular mechanism for the treatment and prevention of hypoxic acute lung injury (ALI) in rats using Huangqi Baihe Granules. The HCP-III laboratory animal low-pressure simulation chamber was utilized to simulate high-altitude environmental exposure and establish an ALI model in rats. The severity of lung damage was evaluated using a battery of tests that included spirometry, a wet/dry lung ratio, H&E staining, and transmission electron microscopy. Using immunofluorescence, the amount of reactive oxygen species (ROS) in lung tissue was determined. Superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and myeloperoxidase (MPO) levels in lung tissue were determined using this kit. Serum levels of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1 beta), and antiinflammatory cytokines like interleukin-10 (IL-10) were measured using an enzyme-linked immunosorbent assay kit. Gene expression changes in lung tissue were identified using transcriptomics, and the relative expression of proteins and mRNA involved in the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB p65)/Nod-like receptor protein 3 (NLRP3) pathway were determined using western blotting and quantitative real-time PCR. HQBHG was shown to enhance lung function considerably, decrease the wet/dry ratio of the lungs, attenuate lung tissue damage, suppress ROS and MDA formation, and increase SOD activity and GSH expression. The research also demonstrated that HQBHG inhibited the activation of the TLR4/NF-κB p65/NLPR3 signaling pathway in lung tissue, reducing the release of downstream pro-inflammatory cytokines. HQBHG exhibits potential therapeutic effects against ALI induced by altitude hypoxia through suppressing oxidative stress and inflammatory response. This suggests it may be a novel drug for treating and preventing ALI. [Display omitted] •HQBHG possesses antioxidant and anti-inflammatory properties, thereby alleviating HH-induced ALI in rats.•HQBHG inhibited lung oxidative stress and alleviated ALI in rats.•HQBHG ameliorated ALI in rats by inhibiting the TLR4/NF-κB/NLRP3 signaling pathway.