Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

During a survey in a tomato field in Luziânia (Goiás State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequenc...

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Published inArchives of virology Vol. 166; no. 11; pp. 3217 - 3220
Main Authors Martins, Thais P., Souza, Tadeu A., da Silva, Patricia S., Nakasu, Erich Y. T., Melo, Fernando L., Inoue-Nagata, Alice K., Nagata, Tatsuya
Format Journal Article
LanguageEnglish
Published Vienna Springer Vienna 01.11.2021
Springer Nature B.V
Subjects
Annotated Sequence Record
Binding sites
Biomedical and Life Sciences
Biomedicine
Cloning
Deoxyribonucleic acid
DNA
DNA sequencing
Genomes
Infectious Diseases
Laboratories
Leaves
Medical Microbiology
Nucleotide sequence
Phylogenetics
Plant viruses
Virology
Viruses
Brazil
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Summary:During a survey in a tomato field in Luziânia (Goiás State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus , named "tomato mottle leaf distortion virus".
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ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-021-05220-w