Anti-inflammatory norabietane diterpenoids from the leaves of Salvia officinalis L

• Published in: Journal of functional foods Vol. 54; pp. 154 - 163
• Main Authors: Li, Lingnan, Wei, Shanshan, Zhu, Tianyu, Xue, Guimin, Xu, Dingqiao, Wang, Wenli, Wang, Xiaobing, Luo, Jianguang, Kong, Lingyi
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.03.2019
• Subjects: anti-inflammatory activity; Bioassay-guided method; C20-norabietane diterpenoids; diterpenoids; drugs; fractionation; herbal tea; leaves; lipopolysaccharides; macrophages; MAPK pathway; mechanism of action; Mediterranean region; Molecular docking; nitric oxide; NO inhibitory effect; sage; Salvia officinalis; spectral analysis; Mediterranean region; MAPK pathway; NO inhibitory effect; Bioassay-guided method; C20-norabietane diterpenoids; Salvia officinalis; Molecular docking
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2019.01.020

[Display omitted] •Eight new and four known norabietane diterpenoids from S. officinalis were isolated.•7 exhibited the highest NO inhibitory effect in LPS-induced RAW264.7 Cells.•The analysis of structure-activity and effect mechanism by the molecular docking.•The mechanism of action on NO inhibitory effect was explored. As an aromatic plant of the Labiatae family, Salvia officinalis L. (sage), is cultured worldwide, especially in Mediterranean region. The leaves of sage are used as a popular herbal tea, drug and seasoning. In the present study, eight novel C20-norabietane diterpenoids (salofficinoids A-H, 1–8), along with four known analogues (9–12) which were responsible for the anti-inflammatory activity, were purified from the sage leaves via bioassay-guided fractionation. Their structures were investigated using extensive spectroscopic analyses. The anti-inflammatory effects of all isolates on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophage cells were assessed. Among them, salofficinoids G (7), which exhibited the most potent NO inhibitory effect, was further evaluated, and its mechanism of action was explored.