Neuropeptide Y inhibits ciliary beat frequency in human ciliated cells via nPKC, independently of PKA
Departments of Medicine, Pediatrics, and Bioengineering, University of Illinois at Chicago and Veterans Affairs Chicago Health Care System, Chicago, Illinois 60612 The intracellular mechanisms whereby the inhibitory neurotransmitter neuropeptide Y (NPY) decreases ciliary beat frequency (CBF) were in...
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Published in | American Journal of Physiology: Cell Physiology Vol. 275; no. 2; pp. C440 - C448 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.08.1998
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Subjects | |
Online Access | Get full text |
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Summary: | Departments of Medicine, Pediatrics, and Bioengineering, University
of Illinois at Chicago and Veterans Affairs Chicago Health Care
System, Chicago, Illinois 60612
The intracellular
mechanisms whereby the inhibitory neurotransmitter neuropeptide Y (NPY)
decreases ciliary beat frequency (CBF) were investigated in cultured
human tracheal and bronchial ciliated cells. CBF was measured by
nonstationary analysis laser light scattering. NPY at 1 and 10 µM
decreased CBF from a baseline of 6.7 ± 0.5 ( n = 12) to 6.1 ± 0.5 ( P < 0.05) and 5.8 ± 0.4 ( P < 0.01) Hz, respectively. Prior
application of PYX-1, an NPY antagonist, prevented the
decreases of CBF induced by both doses of NPY. Two broad protein kinase
C (PKC) kinase inhibitors, staurosporine and calphostin C, also
abolished the NPY-induced decrease in CBF. The NPY-induced decrease in
CBF was abolished by GF 109203X, a novel PKC (nPKC)
isoform inhibitor, whereas this decrease in CBF was not attenuated by
Gö-6976, a specific inhibitor of conventional PKC isoforms.
Because pretreatment with NPY did not block the stimulation of CBF by
forskolin and pretreatment with forskolin did not abolish the
NPY-induced inhibition of CBF, this NPY receptor-mediated signal
transduction mechanism appears to be independent of the adenylate
cyclase-protein kinase A (PKA) pathway. Inhibition of Ca 2+ -ATPase by thapsigargin also
prevented the suppression of CBF induced by subsequent application of
NPY. These novel data indicate that, in cultured human epithelia, NPY
decreases CBF below its basal level via the activation of an nPKC
isoform and Ca 2+ -ATPase,
independent of the activity of PKA. This is consistent with the
proposition that NPY is an autonomic efferent inhibitory neurotransmitter regulating mucociliary transport.
Gö-6976; GF 109203X; neuropeptide Y receptor subtype 1 (Y 1 ); neuropeptide Y receptor subtype 2 (Y 2 ); thapsigargin |
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ISSN: | 0363-6143 0002-9513 1522-1563 |
DOI: | 10.1152/ajpcell.1998.275.2.c440 |