Application of sodium nitroprusside results in distinct antioxidant gene expression patterns in leaves of mature and senescing Medicago truncatula plants

• Published in: Protoplasma Vol. 251; no. 4; pp. 973 - 978
• Main Authors: Fotopoulos, Vasileios, Antoniou, Chrystalla, Filippou, Panagiota, Mylona, Photini, Fasoula, Dionysia, Ioannides, Ioannis, Polidoros, Alexios
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 01.07.2014; Springer Vienna; Springer Nature B.V
• Subjects: antioxidants; Antioxidants - metabolism; Biomedical and Life Sciences; Cell Biology; enzymes; free radicals; gene expression; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; genes; leaves; Life Sciences; Medicago truncatula; Medicago truncatula - drug effects; Medicago truncatula - genetics; Medicago truncatula - metabolism; nitric oxide; nitroprusside; Nitroprusside - pharmacology; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Sciences; Polymorphism, Single Nucleotide - genetics; Reverse Transcriptase Polymerase Chain Reaction; Short Communication; transcription (genetics); Zoology; Antioxidants; Sodium nitroprusside; Oxidative stress; Nitrosative stress
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-013-0573-0

Sodium nitroprusside (SNP) represents one of the most commonly used NO donors in biological sciences, which acts as a signal molecule in plants responsible for the regulation of the expression of many defense-related enzymes. This study attempts to provide novel insight into the effect of application of low (100 μΜ) and high (2.5 mM) concentrations of SNP on antioxidant gene expression (cAPX, GST, FeSOD, CAT, and AOX) in mature (40 day) and senescing (65 day) Medicago truncatula plants. Quantitative real-time RT-PCR suggests that low concentration of SNP applied in mature leaves leads to an overall induction of antioxidant gene expression, while increasing concentration results in suppression of these genes. Conversely, older plants demonstrate a much more variable regulation which appears to be time dependent. The observed transcriptional regulation pattern in mature M. truncatula plants comes in support of the previously documented protective or damaging effect of SNP depending on concentration applied, whereas senescing M. truncatula plants demonstrated a general suppression in antioxidant gene expression levels regardless of SNP concentration, indicative of reduced overall plant defense capacity against free radicals.