Genomic molecular signatures determined characterization of Mycolicibacterium gossypii sp. nov., a fast-growing mycobacterial species isolated from cotton field soil

• Published in: Antonie van Leeuwenhoek Vol. 114; no. 10; pp. 1735 - 1744
• Main Authors: Huang, Rui-Rui, Yang, Shen-Rong, Zhen, Cheng, Ge, Xian-Feng, Chen, Xin-Kai, Wen, Zhi-Qiang, Li, Ya-Nan, Liu, Wen-Zheng
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2021; Springer Nature B.V
• Subjects: Acidic soils; Antimicrobial resistance; Biomedical and Life Sciences; Cardiolipin; Cotton; Deoxyribonucleic acid; DNA; Fatty acids; Genomic analysis; Hybridization; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotides; Original Paper; Phosphatidylethanolamine; Phosphatidylinositol; Phylogenetics; Phylogeny; Plant Sciences; rRNA 16S; Signatures; Soil Science & Conservation; Fast-growing; Cotton field soil; Molecular signatures
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01638-z

A Gram-positive, acid-fast and rapidly growing rod, designated S2-37  T , that could form yellowish colonies was isolated from one soil sample collected from cotton cropping field located in the Xinjiang region of China. Genomic analyses indicated that strain S2-37  T harbored T7SS secretion system and was very likely able to produce mycolic acid, which were typical features of pathogenetic mycobacterial species. 16S rRNA-directed phylogenetic analysis referred that strain S2-37  T was closely related to bacterial species belonging to the genus Mycolicibacterium , which was further confirmed by pan-genome phylogenetic analysis. Digital DNA-DNA hybridization and the average nucleotide identity presented that strain S2-37  T displayed the highest values of 39.1% (35.7–42.6%) and 81.28% with M. litorale CGMCC 4.5724  T , respectively. And characterization of conserved molecular signatures further supported the taxonomic position of strain S2-37  T belonging to the genus Mycolicibacterium . The main fatty acids were identified as C 16:0 , C 18:0 , C 20:3 ω3 and C 22:6 ω3. In addition, polar lipids profile was mainly composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Phylogenetic analyses, distinct fatty aids and antimicrobial resistance profiles indicated that strain S2-37  T represented genetically and phenotypically distinct from its closest phylogenetic neighbour, M. litorale CGMCC 4.5724  T . Here, we propose a novel species of the genus Mycolicibacterium : Mycolicibacterium gossypii sp. nov. with the type strain S2-37  T (= JCM 34327  T  = CGMCC 1.18817  T ).