Galactoglucomannan structure of Arabidopsis seed‐coat mucilage in GDP‐mannose synthesis impaired mutants

• Published in: Physiologia plantarum Vol. 173; no. 3; pp. 1244 - 1252
• Main Authors: Nishigaki, Naho, Yoshimi, Yoshihisa, Kuki, Hiroaki, Kunieda, Tadashi, Hara‐Nishimura, Ikuko, Tsumuraya, Yoichi, Takahashi, Daisuke, Dupree, Paul, Kotake, Toshihisa
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2021; Wiley Subscription Services, Inc
• Subjects: Arabidopsis; Ascorbic acid; Cell walls; Guanosine diphosphate; Mannan; Mannanases; Mannose; Mucilage; Mutants; Mutation; Nucleotides; Oligosaccharides; Polysaccharides; Saccharides; Structural analysis; Substrates; Sugar; Synthesis
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.13519

Cell‐wall polysaccharides are synthesized from nucleotide sugars by glycosyltransferases. However, in what way the level of nucleotide sugars affects the structure of the polysaccharides is not entirely clear. guanosine diphosphate (GDP)‐mannose (GDP‐Man) is one of the major nucleotide sugars in plants and serves as a substrate in the synthesis of mannan polysaccharides. GDP‐Man is synthesized from mannose 1‐phosphate and GTP by a GDP‐Man pyrophosphorylase, VITAMIN C DEFECTIVE1 (VTC1), which is positively regulated by the interacting protein KONJAC1 (KJC1) in Arabidopsis. Since seed‐coat mucilage can serve as a model of the plant cell wall, we examined the influence of vtc1 and kjc1 mutations on the synthesis of mucilage galactoglucomannan. Sugar composition analysis showed that mannose content in adherent mucilage of kjc1 and vtc1 mutants was only 42% and 11% of the wild‐type, respectively, indicating a drastic decrease of galactoglucomannan. On the other hand, structural analysis based on specific oligosaccharides released by endo‐β‐1,4‐mannanase indicated that galactoglucomannan had a patterned glucomannan backbone consisting of alternating residues of glucose and mannose and the frequency of α‐galactosyl branches was also similar to the wild type structure. These results suggest that the structure of mucilage galactoglucomannan is mainly determined by properties of glycosyltransferases rather than the availability of nucleotide sugars.