Investigation of Mating Pheromone-Pheromone Receptor Specificity in Lentinula edodes

• Published in: Genes Vol. 11; no. 5; p. 506
• Main Authors: Kim, Sinil, Ha, Byeongsuk, Kim, Minseek, Ro, Hyeon-Su
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.05.2020; MDPI
• Subjects: Alleles; Amino Acid Sequence; Amino acids; Carboxymethylation; Cell cycle; Clamp connections; Flow cytometry; Fungal Proteins - genetics; Fungal Proteins - metabolism; FUS1 gene; Gene Expression Regulation, Fungal; Genes; Genes, Fungal; Genes, Mating Type, Fungal - genetics; Kinases; Lentinula edodes; Mating; mating pheromone; mating receptor; Mycelium - metabolism; Mycelium - ultrastructure; Peptide Fragments - chemical synthesis; Peptide Fragments - metabolism; Pheromone receptors; Pheromones; Pheromones - chemistry; Pheromones - metabolism; Protein Binding; Proteins; Receptors, Pheromone - genetics; Receptors, Pheromone - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Reproduction - genetics; Saccharomyces cerevisiae; Self-recognition; Shiitake Mushrooms - genetics; Signal Transduction; specificity; Substrate Specificity; Yeast; United States > US; specificity; mating receptor; Lentinula edodes; mating pheromone
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes11050506

The mating-type locus of , a representative edible mushroom, is highly complex because of allelic variations in the mating pheromone receptors ( s) and the mating pheromones ( s) in both the and subloci. The complexity of the mating-type locus, five subloci with five alleles of and nine and three subloci with 3 alleles of and five s, has led us to investigate the specificity of the PHB-RCB interaction because the interaction plays a key role in non-self-recognition. In this study, the specificities of PHBs to RCB1-2 and RCB1-4 from the sublocus and RCB2-1 from the sublocus were investigated using recombinant yeast strains generated by replacing , an endogenous yeast mating pheromone receptor, with the s. Fourteen synthetic PHBs with C-terminal carboxymethylation but without farnesylation were added to the recombinant yeast cells and the PHB-RCB interaction was monitored by the expression of the gene-a downstream gene of the yeast mating signal pathway. RCB1-2 ( ) was activated by PHB1 (4.3-fold) and PHB2 (2.1-fold) from the sublocus and RCB1-4 ( ) was activated by PHB5 (3.0-fold) and PHB6 (2.7-fold) from the sublocus and PHB13 (3.0-fold) from the sublocus. In particular, PHB3 from and PHB9 from showed strong activation of RCB2-1 of the sublocus by 59-fold. The RCB-PHB interactions were confirmed in the monokaryotic S1-10 strain of by showing increased expression of a downstream gene of the mating signal pathway and the occurrence of clamp connections after the treatment of PHBs. These results indicate that a single PHB can interact with a non-self RCB in a sublocus-specific manner for the activation of the mating pheromone signal pathways in .