Suppression of fibroblast metalloproteinases by ajulemic acid, a nonpsychoactive cannabinoid acid
Production of matrix metalloproteinases (MMP) in joint tissue of patients with inflammatory arthritis facilitates cartilage degradation and bone erosion, and leads to joint deformities and crippling. Thus, MMPs are important targets for agents designed to treat inflammatory arthritis. Oral administr...
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Published in | Journal of cellular biochemistry Vol. 100; no. 1; pp. 184 - 190 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.01.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Production of matrix metalloproteinases (MMP) in joint tissue of patients with inflammatory arthritis facilitates cartilage degradation and bone erosion, and leads to joint deformities and crippling. Thus, MMPs are important targets for agents designed to treat inflammatory arthritis. Oral administration of ajulemic acid (AjA), a synthetic, nonpsychoactive cannabinoid acid, prevents joint tissue injury in rats with adjuvant arthritis. AjA binds to and activates PPARγ directly. Therefore, we investigated the influence of AjA on MMP production in human fibroblast‐like synovial cells (FLS), and examined the role of PPARγ in the mechanism of action of AjA. FLS, treated or not with a PPARγ antagonist, were treated with AjA then stimulated with TNFα or IL‐1α. Release of MMPs‐1, 3, and 9 was measured by ELISA. The influence of AjA on MMP‐3 release from stimulated PPARγ positive (PPAR+/−) and PPARγ null (PPAR−/−) mouse embryonic fibroblasts (MEF) was also examined. Addition of AjA to FLS suppressed production of MMPs whether or not PPARγ activation was blocked. Secretion of MMP‐3 was also suppressed by AjA in both TNFα‐ and IL‐1α‐stimulated PPARγ+/− and PPARγ−/− MEF. Suppression of MMP secretion from FLS by AjA appears to be PPARγ independent. Prevention by AjA of joint tissue injury and crippling in the rat adjuvant arthritis model may be explained in large part by inhibition of MMPs. These results suggest that AjA may be useful for treatment of patients with rheumatoid arthritis and osteoarthritis. J. Cell. Biochem. 100: 184–190, 2007. © 2006 Wiley‐Liss, Inc. |
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Bibliography: | ArticleID:JCB21046 ark:/67375/WNG-PRZWGB3S-Z NIH - No. R01 DA13691; No. T32 AR07572 istex:0F962508D3E297E57ED06AE13465B74409760EC9 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0730-2312 1097-4644 |
DOI: | 10.1002/jcb.21046 |