Novel surface expression of reticulocalbin 1 on bone endothelial cells and human prostate cancer cells is regulated by TNF-α

An unbiased cDNA expression phage library derived from bone‐marrow endothelial cells was used to identify novel surface adhesion molecules that might participate in metastasis. Herein we report that reticulocalbin 1 (RCN1) is a cell surface‐associated protein on both endothelial (EC) and prostate ca...

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Published inJournal of cellular biochemistry Vol. 104; no. 6; pp. 2298 - 2309
Main Authors Cooper, Carlton R., Graves, Bianca, Pruitt, Freddie, Chaib, Hassan, Lynch, Jill E., Cox, Andrew Koemeter, Sequeria, Linda, van Golen, Kenneth L., Evans, Angelo, Czymmek, Kirk, Bullard, Rebecca S., Donald, Carlton D., Sol-Church, Katia, Gendernalik, James D., Weksler, Babette, Farach-Carson, Mary C., Macoska, Jill A., Sikes, Robert A., Pienta, Kenneth J.
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.08.2008
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Summary:An unbiased cDNA expression phage library derived from bone‐marrow endothelial cells was used to identify novel surface adhesion molecules that might participate in metastasis. Herein we report that reticulocalbin 1 (RCN1) is a cell surface‐associated protein on both endothelial (EC) and prostate cancer (PCa) cell lines. RCN1 is an H/KDEL protein with six EF‐hand, calcium‐binding motifs, found in the endoplasmic reticulum. Our data indicate that RCN1 also is expressed on the cell surface of several endothelial cell lines, including human dermal microvascular endothelial cells (HDMVECs), bone marrow endothelial cells (BMEC), and transformed human bone marrow endothelial cells (TrHBMEC). While RCN1 protein levels were highest in lysates from HDMVEC, this difference was not statistically significant compared BMEC and TrHBMEC. Given preferential adhesion of PCa to bone‐marrow EC, these data suggest that RCN1 is unlikely to account for the preferential metastasis of PCa to bone. In addition, there was not a statistically significant difference in total RCN1 protein expression among the PCa cell lines. RCN1 also was expressed on the surface of several PCa cell lines, including those of the LNCaP human PCa progression model and the highly metastatic PC‐3 cell line. Interestingly, RCN1 expression on the cell surface was upregulated by tumor necrosis factor alpha treatment of bone‐marrow endothelial cells. Taken together, we show cell surface localization of RCN1 that has not been described previously for either PCa or BMEC and that the surface expression on BMEC is regulated by pro‐inflammatory TNF‐α. J. Cell. Biochem. 104: 2298–2309, 2008. © 2008 Wiley‐Liss, Inc.
Bibliography:NIH-K22 Career-Transition Award - No. 5K22CA971117-3; No. CA096788
NIH Extramural Research Facilities Program - No. C06RR14516; No. RO1-HL55267
istex:81680E954D21F02EBA1FF1A301A502F98E9230C2
University of Delaware
NIHNCRRINBRE - No. RR016472-04
ArticleID:JCB21785
NIH/NCI - No. PO1 CA098912; No. DAMD-17-00-1-0049; No. CA-105435; No. DK63919; No. CA-60948
The University of Michigan Comprehensive Cancer Center - No. CA 46592 (KP)
National Foundation for Cancer Research Center for Metastasis Research (CRC)
Prostate Cancer SPORE - No. P50 CA 69568
ark:/67375/WNG-3TGPVMZH-X
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.21785