New rapid clean-up device for ochratoxin A

• Published in: JSM Mycotoxins Vol. 2003; no. Suppl3; pp. 315 - 318
• Main Authors: BUTTINGER, Gerhard, KNAPP, Hermann, FUCHS, Elisabeth, BINDER, Eva-Maria, KRSKA, Rudolf
• Format: Journal Article
• Language: English
• Published: Japanese Society of Mycotoxicology 2003
• Subjects: food analysis; high performance liquid chromatography; Mycosep; ochratoxin A; sample clean-up
• ISSN: 0285-1466; 1881-0128
• DOI: 10.2520/myco1975.2003.Suppl3_315

High Performance Liquid Chromatography (HPLC) with fluorescence detection (FLD) and Thin Layer Chromatography (TLC) are widely used analytical methods for the determination of Ochratoxin A (OTA). As normally the crude extract cannot be used directly for these analyses, a clean-up step is necessary. Commonly used clean-up procedures comprise immunoaffinity columns (IAC), liquid liquid extraction (LLE) and solid phase extraction (SPE). The performance of a new single step clean-up column Mycosep® 229 Ochra from Romerlabs® was tested in matrices often contaminated with OTA like green coffee beans, raisins and various kinds of cereals. In all these matrices an average recovery of more than 90% (range 2.6-92μg/kg; n=24) of the added OTA could be obtained. Additionally, a certified reference material (CRM), OTA in wheat, was tested with the column. An average recovery of 96.2% (n=9) was achieved for the CRM proving the applicability and high accuracy of the developed method.