Persistence of proinflammatory response after severe respiratory syncytial virus disease in children

• Published in: Journal of allergy and clinical immunology Vol. 119; no. 6; pp. 1547 - 1550
• Main Authors: Bermejo-Martin, Jesus F., MD, PhD, Garcia-Arevalo, M. Carmen, MSc, Alonso, Ana, MD, De Lejarazu, Raul O., MD, PhD, Pino, Maria, MD, Resino, Salvador, PhD, Tenorio, Alberto, MD, Bernardo, David, MSc, Leon, Alberto J., PhD, Garrote, Jose A., MD, PhD, Ardura, Julio, MD, PhD, Dominguez-Gil, Marta, MSc, Eiros, Jose M., MD, PhD, Blanco-Quiros, Alfredo, MD, PhD, Munoz-Fernandez, M. Angeles, PhD, Kelvin, David J., PhD, Arranz, Eduardo, MD, PhD
• Format: Journal Article
• Language: English
• Published: United States Mosby, Inc 01.06.2007; Elsevier Limited
• Subjects: Allergy and Immunology; Chemokines; Chemokines - biosynthesis; Chemokines - genetics; Colleges & universities; Gene Expression Regulation, Viral - immunology; Humans; Infant; Infections; Inflammation Mediators - metabolism; Proteins; Respiratory Syncytial Virus Infections - immunology; Respiratory Syncytial Virus Infections - metabolism; Respiratory Syncytial Virus Infections - virology; Respiratory Syncytial Viruses - immunology; Respiratory Tract Infections - immunology; Respiratory Tract Infections - metabolism; Vascular endothelial growth factor
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2007.03.014

Limits of detection were calculated, considering a detectable signal as greater than 2 SDs above blank (isotonic solution ClNa 0.9%): eotaxin, 4.57 pg/mL; GM-CSF, 18.38 pg/mL; IL-1 receptor antagonist (IL-1RA), 2.17 pg/mL; IL-5, 0.27 pg/mL; IL-9, 5.85 pg/mL; IL-13, 0.26 pg/mL; IL-1β, 1.93 pg/mL; monocyte chemoattractant protein 1 (MCP-1), 9.2 pg/mL; platelet-derived growth factor (PDGF-BB), 2.19 pg/mL; vascular endothelial growth factor (VEGF), 4.4 pg/mL; human fibroblast growth factor-basic (FGF-b), 6.15 pg/mL; IL-2, 1.49 pg/mL; IL-6, 3.81 pg/mL; IL-10, 0.46 pg/mL; IL-15, 6.28 pg/mL; IL-8, 5.52 pg/mL; macrophage inflammatory protein 1α (MIP-1α), 1.12 pg/mL; RANTES, 1.18 pg/mL; granulocyte colony-stimulating factor, 0.42 pg/mL; IFN-γ, 4.14 pg/mL; IL-4, 1.68 pg/mL; IL-7, 0.65 pg/mL; IL-12p70, 2.41 pg/mL; IL-17, 1.9 pg/mL; IFN-inducible protein 10, 2.51 pg/mL; MIP-1β, 6.41 pg/mL; and TNF-α, 37.87 pg/mL. Supplementary data Supplementary data Supplementary data associated with this article can be found in the online version, at doi:10.1016/j.jaci.2007.03.014  Admission (A), n = 37 Discharge (D), n = 37 One month (M), n = 23 Control (C), n = 27 A/D P value A/D/M P value A/M P value D/M P value IL-1RA 6886 (13,230)[low *] 4819 (5558)[low *] 4501 (13,842) 2825 (4189) .483 .840 -- -- IL-5 0.7 (0.5)[low *] 0.4 (0.2)[low *] 0.6 (0.8)[low *] 0.3 (0.3) .018[dagger] .160 -- -- IL-13 3.7 (3.9)[low *] 2.1 (1.7) 1.6 (1.2) 1.3 (2.0) .000[dagger] .070 -- -- IL-1β 422.0 (1030)[low *] 226.9 (597.1)[low *] 231.7 (1337.3)[low *] 32.6 (176.1) .267 .840 -- -- FGF-b 8.3 (14.6)[low *] 6.2 (0.0) 6.2 (0.0) 6.2 (0.0) .000[dagger] .004[double dagger] .022§ .917 IFN-γ 103.4 (45.1)[low *] 63.1 (55.2)[low *] 54.3 (41.4)[low *] 22.4 (59.3) .005[dagger] .066 -- -- MCP-1 9.2 (18.3)[low *] 9.2 (3.2) 9.2 (15.5) 9.2 (0.0) .362 .545 -- -- PDGF-BB 17.4 (19.9)[low *] 13.6 (12.7)[low *] 10.8 (13.8) 4.1 (6.6) .394 .119 -- -- IL-2 4.0 (4.1)[low *] 2.4 (2.5)[low *] 1.8 (3.2)[low *] 1.5 (0.0) .004[dagger] .124 -- -- IL-6 198.5 (424.8)[low *] 108.8 (301.7)[low *] 30.0 (38.0)[low *] 8.0 (37.6) .118 .004[double dagger] .006§ .009|| IL-10 11.8 (18.0)[low *] 4.6 (6.3)[low *] 3.8 (12.1)[low *] 1.2 (2.8) .000[dagger] .003[double dagger] .089 .101 IP-10 12,201 (18,148)[low *] 3507 (7227)[low *] 5316 (7535)[low *] 2510 (3961.5) .004[dagger] .070 -- -- IL-8 7898 (6246)[low *] 3999 (6177)[low *] 5007 (7293)[low *] 573.0 (3221.4) .088 .599 -- -- G-CSF 1317 (1979)[low *] 1354 (1994)[low *] 608.9 (1532.2)[low *] 155.6 (338.0) .922 .154 -- -- MIP-1α 48.6 (109.7)[low *] 42.2 (81.0)[low *] 17.6 (53.8)[low *] 2.0 (17.1) .126 .019[double dagger] .144 .136 RANTES 71.5 (100.8) 47.9 (87.5) 49.1 (59.4) 47.3 (118.5) .202 .104 -- -- IL-4 2.3 (1.6)[low *] 1.7 (0.4) 1.7 (0.6)[low *] 1.7 (0.0) .008[dagger] .116 -- -- IL-7 9.3 (7.1) 9.0 (7.3) 10.7 (14.2) 4.0 (13.6) .780 .401 -- -- IL-12p70 3.8 (3.0)[low *] 2.8 (1.3)[low *] 2.6 (1.8)[low *] 2.4 (0.1) .006[dagger] .034[double dagger] .012§ .983 TNF-α 103.1 (1140)[low *] 37.9 (130)[low *] 37.9 (45)[low *] 37.9 (0.0) .018[dagger] .219 -- -- Eotaxin 34.6 (35.8) 42.9 (47.9)[low *] 55.2 (68.8)[low *] 25.6 (38.2) .167 .018[double dagger] .006§ .191 IL-17 8.5 (8.1)[low *] 4.3 (5.2)[low *] 3.5 (6.1)[low *] 1.9 (2.3) .001[dagger] .103 -- -- MIP-1β 676.3 (1448)[low *] 597.8 (904)[low *] 155.3 (407.8)[low *] 30.1 (109.7) .129 .005[double dagger] .045§ .036|| VEGF 1370 (1666)[low *] 1016 (1193)[low *] 1169 (2875)[low *] 377.5 (921.1) .126 .738 -- -- Table I Comparison of immune mediators levels over time Results are expressed as median + interquartile range.IP-10, IFN-inducible protein 10; G-CSF, granulocyte colony-stimulating factor.