A temporal analysis of testosterone-induced changes in electric organs and electric organ discharges of mormyrid fishes

• Published in: Journal of neurobiology Vol. 20; no. 7; p. 619
• Main Authors: Freedman, E G, Olyarchuk, J, Marchaterre, M A, Bass, A H
• Format: Journal Article
• Language: English
• Published: United States 01.10.1989
• Subjects: Action Potentials - drug effects; Animals; Electric Fish - physiology; Electric Organ - cytology; Electric Organ - drug effects; Electric Organ - physiology; Female; Male; Methyltestosterone - pharmacology; Testosterone - analogs & derivatives; Testosterone - pharmacology
• ISSN: 0022-3034
• DOI: 10.1002/neu.480200703

The electric organ discharge (EOD) of several species of mormyrid fishes within the genus Brienomyrus is sexually dimorphic during the breeding season: the duration of the male's EOD is much longer than the duration of the female's (for a review see Hopkins, 1986). The mormyrid used here, Brienomyrus sp., exhibits similar alterations in the duration of the triphasic EOD after treatment with testosterone, as do other members of this genus (for reviews see Bass, 1986a,b). In this experiment, animals were intraperitoneally implanted with pellets of either 11-ketotestosterone or 17 a-methyltestosterone, and the time course of the changes in the duration of each of the three phases of the EOD were quantified. Additionally, the time course of changes in the morphology of the electric organ, after testosterone treatment, was also quantified using electron microscopic techniques. The results suggest that the change in the duration of the first phase of the EOD is due exclusively to the change in the thickness of the electrocyte body: this is consistent with a model proposed by Bennett and Grundfest (1961) for the electrogenesis of a triphasic EOD. Changes in the duration of the second and third phases of the EOD are highly correlated with the changes in the surface area of the posterior and anterior faces of the electrocyte, respectively. The results support the hypothesis that gonadal steroid hormone-induced changes in the EOD are due to structural changes in the electrocyte's membranes, and that all of the observed changes in the discharge of this system can be explained by the action of steroid hormones on the peripheral target cells (electrocytes).