NAD+ Modulates the Proliferation and Differentiation of Adult Neural Stem/Progenitor Cells via Akt Signaling Pathway

• Published in: Cells (Basel, Switzerland) Vol. 11; no. 8; p. 1283
• Main Authors: Huang, Xiaoli, Guo, Hongfeng, Cheng, Xuejun, Zhang, Jinyu, Qu, Wenzheng, Ding, Qianyun, Sun, Qihang, Shu, Qiang, Li, Xuekun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.04.2022; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; Adenine; adult neural stem/progenitor cells; Akt pathway; AKT protein; Alzheimer's disease; Antibodies; Apoptosis; Cell Proliferation; Citric acid; differentiation; DNA methylation; Gene expression; NAD; NAD - metabolism; Neural stem cells; Neural Stem Cells - metabolism; Neurogenesis; Phosphatidylinositol 3-Kinases - metabolism; Progenitor cells; proliferation; Proto-Oncogene Proteins c-akt - metabolism; Senescence; Signal transduction; Signal Transduction - physiology; Transcriptomes; United States > US; China; Fiji; NAD; adult neural stem/progenitor cells; differentiation; Akt pathway; proliferation
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells11081283

Nicotinamide adenine dinucleotide hydrate (NAD+) acts as the essential component of the tricarboxylic citric acid (TCA) cycle and has important functions in diverse biological processes. However, the roles of NAD+ in regulating adult neural stem/progenitor cells (aNSPCs) remain largely unknown. Here, we show that NAD+ exposure leads to the reduced proliferation and neuronal differentiation of aNSPCs and induces the apoptosis of aNSPCs. In addition, NAD+ exposure inhibits the morphological development of neurons. Mechanistically, RNA sequencing revealed that the transcriptome of aNSPCs is altered by NAD+ exposure. NAD+ exposure significantly decreases the expression of multiple genes related to ATP metabolism and the PI3k-Akt signaling pathway. Collectively, our findings provide some insights into the roles and mechanisms in which NAD+ regulates aNSPCs and neuronal development.