Interleukin 20 protein locates to distinct mononuclear cells in psoriatic skin
We previously demonstrated that mRNA for the pro‐inflammatory cytokine interleukin 20 (IL‐20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin (LS). Here, we describe the distribution of IL‐20 protein and the identity of the IL‐20‐positive cells in LS. We found that the main p...
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Published in | Experimental dermatology Vol. 23; no. 5; pp. 349 - 351 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Denmark
Blackwell Publishing Ltd
01.05.2014
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Subjects | |
Online Access | Get full text |
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Summary: | We previously demonstrated that mRNA for the pro‐inflammatory cytokine interleukin 20 (IL‐20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin (LS). Here, we describe the distribution of IL‐20 protein and the identity of the IL‐20‐positive cells in LS. We found that the main part of IL‐20 immunoreactivity is present in mononuclear cells of the dermal papillae, and that the IL‐20‐positive cells located in the papillae were langerin+, CD1a+, CD4+ and CD303+. These cells might be immature dendritic cell. In situ hybridization for IL‐20 mRNA on non‐LS, ex vivo stimulated with IL‐1β revealed a colocalization between IL‐20 mRNA and the keratinocyte marker CK14. No IL‐20 mRNA was detected in the dermal mononuclear cells. Our results suggest that IL‐20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells. Our study supports that IL‐20 is involved in the pathogenesis of psoriasis. |
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Bibliography: | Figure S1. (a-c) Specificity of the antibody was verified in sections of paraffin-embedded HEK cells transiently transfected with human IL-20 (a, b) and IL-19 (c), respectively. The sections were subjected to immunofluorescence; (a) HEK cells transfected with human IL-20 stained with polyclonal rabbit anti-IL-20 and detected with Alexa 488 (green); (b) HEK cells transfected with human IL-20 and stained with a pre-immune serum control from the rabbit in which the polyclonal rabbit anti-IL-20 was generated and detected with Alexa 488 (green); (c) HEK cells transfected with human IL-19 were stained with polyclonal rabbit anti-IL-20, and the nuclei were marked with Hoechst (blue). Stained for IL-20 (green); (d-g) localization of IL-20 protein in lesional psoriatic skin (LS). Paraffin-embedded punch biopsies from LS were subjected to immunofluorescence. (d) Stained for IL-20 (green); (e) same tissue as (d) stained with pre-immune-rabbit serum; (f) stained for IL-20 (green) and nuclear DNA (blue); (g) same tissue as (f), the anti-IL-20 has been pre-absorbed with antigen (IL-20), also stained for nuclear DNA (blue).Figure S2. Immunofluorescence stainings for IL-20 and double immunofluorescence stainings for IL-20 and selected CD markers in untreated lesional psoriatic skin.Figure S3. Effect of treatment with calcipotriol (10 patients, mild-to-moderate psoriasis) left panel, or cyclosporin A (10 patients, moderate-severe psoriasis) right panel, before (day 0) and after treatment for 14 and 28 days and compared with non-lesional skin also at day 0.Data S1. Experimental design.Table S1. Antibodies used for staining paraffin-embedded punch biopsies. ark:/67375/WNG-5HXZ4ZBB-H The Danish Psoriasis Foundation The Aage Bang Foundation istex:52AB00BED8B36F7EC15A6CBFAD996D50BF723BB3 ArticleID:EXD12371 SourceType-Other Sources-1 ObjectType-Article-2 content type line 63 ObjectType-Correspondence-1 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0906-6705 1600-0625 |
DOI: | 10.1111/exd.12371 |